School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Sydney, NSW 2006, Australia.
IBG-2: Plant Sciences, Forschungszentrum Jülich, Jülich, Germany.
J Exp Bot. 2022 Jun 2;73(11):3774-3786. doi: 10.1093/jxb/erac097.
The development of reproductive tissues determines plant fecundity and yield. Loading of resources into the developing reproductive tissue is thought to be under the co-limiting effects of source and sink strength. The dynamics of this co-limitation are unknown, largely due to an inability to measure the flux of resources into a developing sink. Here we use nuclear magnetic resonance (NMR) sensors to measure sink strength by quantifying rates of pod dry matter accumulation (pod loading) in Phaseolus vulgaris at 13-min intervals across the diel period. Rates of pod loading showed contrasting variation across light and dark periods during the onset of water deficit. In addition, rates of pod loading appeared decoupled from net photosynthetic rates when adjusted to the plant scale. Combined, these observations illustrate that the rate of pod development varies under water limitation and that continuous, non-invasive methodologies to measure sink strength provide insight into the governing processes that determine the development of reproductive tissues.
生殖组织的发育决定了植物的繁殖力和产量。人们认为，将资源加载到正在发育的生殖组织中受到源和汇强度的共同限制作用。这种共同限制的动态尚不清楚，这主要是因为无法测量资源流入发育中的汇的通量。在这里，我们使用核磁共振（NMR）传感器通过量化在整个昼夜期间每隔 13 分钟在 Phaseolus vulgaris 中豆荚干物质积累（豆荚加载）的速率来通过量化豆荚干物质积累的速率来衡量汇的强度。在开始缺水时，豆荚加载的速率在光照和黑暗期表现出截然不同的变化。此外，当调整到植物尺度时，豆荚加载的速率似乎与净光合速率脱钩。综上所述，这些观察结果表明，在水分限制下，豆荚发育的速度会发生变化，并且连续的、非侵入性的测量汇强度的方法为确定生殖组织发育的控制过程提供了深入的了解。
