Dehkordi Reza Solymani, Mirzaei Abdolah, Boostani Alidad
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Anim Reprod Sci. 2022 Apr;239:106969. doi: 10.1016/j.anireprosci.2022.106969. Epub 2022 Mar 15.
The aim of this study was to evaluate reproductive efficiency of ewes following a short-term treatment with progesterone (P4) plus human chorionic gonadotropin (hCG) injections. In Exp 1, ewes (n = 65) were used during the non-breeding season and received P4, three times every other day (n = 22, 3PHCG, IM) or a single dose of P4 (n = 21, PHCG). We injected hCG in all ewes 24 h after the P4 treatment, and distilled water in control group (n = 22). Blood collection were performed on days -13, -6 and + 8 relative to ram release. In Exp 2, during the breeding season, ewes (n = 66) were assigned to the 3PHCG (n = 33) and control (n = 33) groups described for Exp 1, and subsequently received PGF2α 48 h before ram release. Pregnancy diagnosis was performed on day 42 after the ram release which was done 24 h after the hCG injection. In Exp 1, Estrus, pregnancy rate at induced estrus, and lambing rates at the first cycle were greater in 3PHCG (81.8%, 59.1% and 54.5%) group compared with the PHCG (38.1%, 23.8% and 19%) and control (0%, 0% and 0%) groups (P < 0.05). In Exp 2, Estrus, pregnancy rate at induced estrus, and lambing rates at the first cycle were greater in 3PHCG (75.8%, 57.6% and 51.5%) group compared with the control (39.4%, 30.3% and 27.3%) groups (P < 0.05). To conclude, hCG injection 24 h after P4 administration is an effective protocol to induce fertile estrus during non-breeding season, and synchronize estrus and lambing of ewe in the breeding season.
本研究的目的是评估母羊在接受短期孕酮（P4）加人绒毛膜促性腺激素（hCG）注射治疗后的繁殖效率。在实验1中，在非繁殖季节使用母羊（n = 65），每隔一天接受三次P4（n = 22，3PHCG，肌肉注射）或单剂量P4（n = 21，PHCG）。在P4治疗24小时后，对所有母羊注射hCG，对照组（n = 22）注射蒸馏水。在与公羊放入相关的第-13天、-6天和+8天进行采血。在实验2中，在繁殖季节，将母羊（n = 66）分配到实验1中描述的3PHCG组（n = 33）和对照组（n = 33），随后在公羊放入前48小时接受前列腺素F2α（PGF2α）。在hCG注射24小时后进行公羊放入，在公羊放入后第42天进行妊娠诊断。在实验1中，3PHCG组（81.8%、59.1%和54.5%）的发情率、诱导发情时的妊娠率和第一个周期的产羔率高于PHCG组（38.1%、23.8%和19%）和对照组（0%、0%和0%）（P < 0.05）。在实验2中，3PHCG组（75.8%、57.6%和51.5%）的发情率、诱导发情时的妊娠率和第一个周期的产羔率高于对照组（39.4%、30.3%和27.3%）（P < 0.05）。总之，P4给药24小时后注射hCG是在非繁殖季节诱导可育发情以及在繁殖季节使母羊发情和产羔同步的有效方案。
