Boscos C M, Samartzi F C, Dellis S, Rogge A, Stefanakis A, Krambovitis E
Department of Clinical Studies, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Greece.
Theriogenology. 2002 Oct 15;58(7):1261-72. doi: 10.1016/s0093-691x(02)01040-3.
The main objective of this study was to investigate the effectiveness of certain progestagen-gonadotrophin treatments on synchronization of estrus in sheep. In Experiment I, 30 Chios ewes were treated at the beginning of the breeding season with medroxyprogesterone acetate (MAP) intravaginal sponges for 12 days and a single i.m. treatment of either FSH (Group 1,10 IU, n = 8; Group 2, 5 IU, n = 8; Group 3, 2.5 IU, n = 8) or eCG (Group 4, 400 IU, n = 6) at the time of sponge removal. Ten days after sponge removal laparotomy was performed to record ovarian response. Clinical estrus was observed in more (though not at a significant level) FSH treated than eCG treated sheep (62.5% versus 33.3%). Administration of 400 IU eCG resulted in the highest mean number of CL perewe ovulating (2.8 +/- 0.2), with administration of 10 IU FSH producing the next best results (2.1 +/- 0.3). Statistically significant differences in the mean number of CL per ewe ovulating were found only between ewes in Group 3 (1.7 +/- 0.4) and Group 4 (2.8 +/- 0.2) (P < 0.05). In Experiment II, 53 Chios and 30 Berrichon ewes were treated during the mid-breeding season with MAP intravaginal sponges for 12 days and a single i.m. treatment of either 10 IU FSH (27 Chios and 16 Berrichon ewes) or 400 IU eCG (26 Chios and 14 Berrichon ewes), at the time of sponge removal. Ewes that were in estrus on Days 2-4 and 19-23 after sponge removal were mated to fertile rams. No significant differences were recorded between treatment or breed groups in the proportions of ewes observed in estrus after treatment. In the Berrichon breed, FSH administration resulted in higher lambing rates (93.8% versus 57.1%, P < 0.05) and higher mean number of lambs born per ewe exposed to rams (1.4 +/- 0.2 versus 0.8 +/- 0.2, P < 0.05) than that of eCG. After treatment with eCG, the mean number of lambs born per ewe exposed to rams was higher in the Chios than the Berrichon breed (1.4 +/- 0.2 versus 0.8 +/- 0.2, P < 0.05). After treatment with FSH, the lambing rate was higher in the Berrichon than the Chios breed (93.8% versus 63.0%, P < 0.05). In conclusion, a single FSH treatment (5 or 10 IU) at the end of progestagen treatment appears to be more effective than eCG for the induction of synchronized estrus in sheep at the beginning of the breeding season, with no cases of abnormal ovarian response observed. During the mid-breeding season FSH (10 IU) appears to be equally as effective as eCG (400 IU) in respect of lambing rate and mean number of lambs born per ewe.
本研究的主要目的是调查某些孕激素 - 促性腺激素处理对绵羊发情同步化的有效性。在实验I中，30只希俄斯母羊在繁殖季节开始时用醋酸甲羟孕酮（MAP）阴道海绵栓处理12天，并在取出海绵栓时进行一次肌肉注射FSH（第1组，10 IU，n = 8；第2组，5 IU，n = 8；第3组，2.5 IU，n = 8）或eCG（第4组，400 IU，n = 6）。取出海绵栓10天后进行剖腹术以记录卵巢反应。接受FSH处理的母羊中观察到发情的数量比接受eCG处理的母羊更多（尽管未达到显著水平）（62.5% 对33.3%）。给予400 IU eCG导致每只排卵母羊的平均黄体数最高（2.8 ± 0.2），给予10 IU FSH产生的效果次之（2.1 ± 0.3）。仅在第3组（1.7 ± 0.4）和第4组（2.8 ± 0.2）的母羊之间发现每只排卵母羊的平均黄体数存在统计学显著差异（P < 0.05）。在实验II中，53只希俄斯母羊和30只贝里洪母羊在繁殖季节中期用MAP阴道海绵栓处理12天，并在取出海绵栓时进行一次肌肉注射10 IU FSH（27只希俄斯母羊和16只贝里洪母羊）或400 IU eCG（26只希俄斯母羊和14只贝里洪母羊）。在取出海绵栓后第2 - 4天和第19 - 23天发情的母羊与可育公羊交配。处理后观察到发情的母羊比例在处理组或品种组之间没有显著差异。在贝里洪品种中，给予FSH导致产羔率更高（93.8% 对57.1%，P < 0.05），并且每只与公羊接触的母羊所产羔羊的平均数量更高（1.4 ± 0.2对0.8 ± 0.2，P < 0.05）比给予eCG的情况。给予eCG处理后，每只与公羊接触的希俄斯母羊所产羔羊的平均数量高于贝里洪品种（1.4 ± 0.2对0.8 ± 0.2，P < 0.05）。给予FSH处理后，贝里洪品种的产羔率高于希俄斯品种（93.8% 对63.0%，P < 0.05）。总之，在孕激素处理结束时单次给予FSH（5或10 IU）在繁殖季节开始时诱导绵羊同步发情似乎比eCG更有效，未观察到异常卵巢反应的情况。在繁殖季节中期，就产羔率和每只母羊所产羔羊的平均数量而言，FSH（10 IU）似乎与eCG（400 IU）同样有效。
