Zhang Ling, Chen Jiasheng, Zhang Xinyue, Wang Yurun, Cen Jinfeng, Shi Guiyan, Sun Mingxue, Wang Xianyou, Meng Wenqi, Xiao Kai
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, Xuzhou 221002, China.
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, Xuzhou 221002, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jul 5;275:121171. doi: 10.1016/j.saa.2022.121171. Epub 2022 Mar 18.
Nerve agents are highly toxic chemical warfare agents that are easy to synthesize and have recently been applied many times in local wars and terrorist attacks. Fluorescent probes have been widely used in life science and medical research due to their features of short reaction time, high sensitivity and good selectivity. Herein, two fluorescent compounds, NMU-1 and NMU-2, were synthesized for the selective detection of nerve agents. NMU-1 exhibited good detection performance for nerve agents. With increasing nerve agent concentration, the fluorescence signal of NMU-1 at 498 nm gradually decreased with an excellent linear relationship. NMU-1 exhibited a low LOD (4.6 μM for DCP and 8.41 μM for soman), a rapid response (less than 3 min) and a large Stokes shift (98 nm) along with obvious color changes. Due to its high sensitivity and good selectivity, NMU-1 was successfully applied to image nerve agents in living PC12 cells. Furthermore, NMU-1 was used as a key element to develop chemical warfare agent test paper, which exhibited significant fluorescent changes under hand-held 365-nm UV light upon contact with nerve agents.
神经毒剂是剧毒化学战剂，易于合成，近年来在局部战争和恐怖袭击中多次被使用。荧光探针因其反应时间短、灵敏度高、选择性好等特点，在生命科学和医学研究中得到了广泛应用。在此，合成了两种荧光化合物NMU-1和NMU-2用于神经毒剂的选择性检测。NMU-1对神经毒剂表现出良好的检测性能。随着神经毒剂浓度的增加，NMU-1在498nm处的荧光信号逐渐降低，呈现出良好的线性关系。NMU-1具有低检测限（对二氯磷酸酯为4.6μM，对梭曼为8.41μM）、快速响应（小于3分钟）、大斯托克斯位移（98nm）以及明显的颜色变化。由于其高灵敏度和良好的选择性，NMU-1成功应用于对活PC12细胞中的神经毒剂进行成像。此外，NMU-1被用作开发化学战剂试纸的关键元件，该试纸在与神经毒剂接触后，在手持365nm紫外光下会呈现出显著的荧光变化。
