Wagner B, Schmidt K H, Wagner M, Köhler W
Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Jul;261(4):432-46. doi: 10.1016/s0176-6724(86)80075-x.
Using a phagocytosis assay (Leijh 1980, Infect. Immun. 30, 421), determination of chemiluminescence, and transmission electron microscopy, the influence of the binding of albumin to M protein-positive group A streptococci on their phagocytosis by human polymorphonuclear leukocytes (PMNL) was investigated. Coating of streptococci with albumin in the absence of other serum components enhanced phagocytosis but not killing of the bacteria. Complement had no additional effect on engulfment. Fibrinogen reduced the enhancing effect of albumin. Albumin had no additional effect on the high phagocytosis rate of an M-protein-negative strain. It could be demonstrated that human PMNL bound human serum albumin-gold conjugate. The results are discussed with regard to the role of plasma proteins in the phagocytosis of streptococci under in vivo-conditions.
利用吞噬作用测定法（Leijh，1980年，《感染与免疫》30卷，421页）、化学发光测定法以及透射电子显微镜，研究了白蛋白与M蛋白阳性A组链球菌结合对其被人多形核白细胞（PMNL）吞噬的影响。在无其他血清成分的情况下，用白蛋白包被链球菌可增强吞噬作用，但对细菌的杀伤作用无增强效果。补体对吞噬作用无额外影响。纤维蛋白原降低了白蛋白的增强作用。白蛋白对M蛋白阴性菌株的高吞噬率无额外影响。可以证明人PMNL能结合人血清白蛋白-金复合物。结合体内条件下血浆蛋白在链球菌吞噬作用中的作用对结果进行了讨论。
