NGF/p75 在精子细胞中的相互作用的影响：兔模型。
The Effect of Interaction NGF/p75 in Sperm Cells: A Rabbit Model.
机构信息
Department of Agricultural, Environmental and Food Science, University of Perugia, Borgo XX Giugno 74, 06100 Perugia, Italy.
Department of Medicine, Section of Human, Clinical and Forensic Anatomy, School of Medicine, University of Perugia, P.le Lucio Severi, 1, Sant'Andrea delle Fratte, 06132 Perugia, Italy.
出版信息
Cells. 2022 Mar 18;11(6):1035. doi: 10.3390/cells11061035.
BACKGROUND
Nerve Growth Factor (NGF) plays an important role in the reproductive system through its receptor's interaction (p75). This paper aims to analyze the impact of NGF p75 in epididymal and ejaculated rabbit semen during in vitro sperm storage.
METHODS
Semen samples from 10 adult rabbit bucks were collected four times (n = 40) and analyzed. NGF was quantified in seminal plasma, and the basal expression of p75 in sperm was established (time 0). Moreover, we evaluated p75, the apoptotic rates, and the main sperm parameters, at times 2-4 and 6 h with or without the administration of exogenous NGF.
RESULTS
Based on the level of p75, we defined the threshold value (25.6%), and sperm were divided into High (H) and Normal (N). During sperm storage, p75 of H samples significantly modulated some relevant sperm parameters. Specifically, comparing H samples with N ones, we observed a reduction in motility and non-capacitated cell number, together with an increased percentage of dead and apoptotic cells. Notably, the N group showed a reduction in dead and apoptotic cells after NGF treatment. Conversely, the NGF administration on H sperm did not change either the percentage of dead cells or the apoptotic rate.
CONCLUSION
The concentration of p75 on ejaculated sperm modulates many semen outcomes (motility, apoptosis, viability) through NGF interaction affecting the senescence of sperm.
背景
神经生长因子（NGF）通过其受体的相互作用（p75）在生殖系统中发挥重要作用。本文旨在分析 NGF p75 在体外精子储存过程中对附睾和射出的兔精液的影响。
方法
从 10 只成年公兔中采集精液样本四次（n = 40）并进行分析。测定精液中的 NGF 含量，并建立精子中 p75 的基础表达（时间 0）。此外，我们在 2-4 小时和 6 小时评估了有无外源性 NGF 处理时 p75、凋亡率和主要精子参数。
结果
根据 p75 的水平，我们定义了阈值（25.6%），并将精子分为高（H）和正常（N）两组。在精子储存过程中，H 组样本的 p75 显著调节了一些相关的精子参数。具体来说，与 N 组相比，我们观察到 H 组的运动能力和非获能细胞数量减少，而死亡和凋亡细胞的比例增加。值得注意的是，NGF 处理后 N 组的死亡和凋亡细胞数量减少。相反，NGF 处理对 H 精子的死亡细胞比例或凋亡率没有影响。
结论
射出精液中 p75 的浓度通过 NGF 相互作用调节许多精液结果（活力、凋亡、活力），从而影响精子的衰老。
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