Evaluation of Antimicrobial Activity in the Extract of Defatted Fed Organic Waste Feed Containing Fermented Effective Microorganisms.

(black soldier fly) larvae (HIL) are considered useful industrial insects for the production of feed for livestock, eco-friendly fertilizer from organic wastes, and biodiesel. Therefore, we evaluated the antimicrobial activity in the extract of crude-oil-extracted crushed HIL powder prepared from HIL fed organic waste containing fermented effective micro-organisms for biodiesel production. The result showed that antimicrobial activity was not fully induced in HIL fed -containing feed. In contrast, increased antimicrobial activity was observed in defatted HIL extract prepared from crude-oil-extracted crushed HIL powder. We found that the extract effectively inhibited the growth of pathogens and antimicrobial-peptide-resistant bacteria, such as three kinds of species, and , , , , and , with a minimum inhibitory concentration of 200-1000 µg/100 µL. Furthermore, no cytotoxicity to CaCO-2 human intestinal cells was observed in the extract. We also found that the production fee of extract equivalent to the antimicrobial activity of melittin was approximately 25-fold less than the production fee of melittin. Therefore, the results demonstrate that crude-oil-extracted crushed HIL powder prepared from HIL fed organic waste containing fermented effective micro-organisms for biodiesel production should be used as the feedstock for synthetic, preservative-free livestock feed and food additives. Taken together, the present study supports the usefulness of HIL as an eco-friendly feedstock in the biodiesel, agricultural, food, and feed industries.