Winiarczyk Dagmara, Winiarczyk Mateusz, Michalak Katarzyna, Winiarczyk Stanisław, Adaszek Łukasz
Department of Internal Diseases of Small Animals, University of Life Sciences of Lublin, 20-400 Lublin, Poland.
Department of Vitreoretinal Surgery, Medical University of Lublin, 20-059 Lublin, Poland.
Animals (Basel). 2022 Mar 16;12(6):748. doi: 10.3390/ani12060748.
In this study we aimed to analyze the protein composition of the urine collected from the healthy animals and compare it to the two diabetic groups (DM I normoalbuminuric diabetic dogs; DM II diabetic dogs with microalbuminuria). We tried to identify potential urinary proteins which could be up- or downregulated in diabetic patients even before the appearance of microalbuminuria. Methods: After obtaining urine, we performed two-dimensional electrophoresis, followed by Delta2D software analysis, which allowed for selection and identification with MALDI-TOF spectrometry, statistically significant differentially expressed proteins. Our study revealed 286 common protein spots on 2D gels from the diabetic and control group. From these proteins five were positively identified by MALDI-TOF MS. To further evaluate the five differentiating proteins, the Panther program was used to assign them to appropriate biological process. Conclusion: Significant number of identified proteins play a role in intracellular signaling-vesicle formation, bonding, transport through membranes. This may suggest that first signs of kidney diabetic cellular impairment may be seen in the urine composition before any clinical signs occur.
在本研究中，我们旨在分析从健康动物收集的尿液的蛋白质组成，并将其与两个糖尿病组（I型正常白蛋白尿糖尿病犬；II型微量白蛋白尿糖尿病犬）进行比较。我们试图鉴定出在糖尿病患者出现微量白蛋白尿之前可能上调或下调的潜在尿蛋白。方法：获取尿液后，我们进行二维电泳，随后进行Delta2D软件分析，该分析允许通过基质辅助激光解吸电离飞行时间质谱法选择和鉴定具有统计学意义的差异表达蛋白。我们的研究在糖尿病组和对照组的二维凝胶上发现了286个常见蛋白点。通过基质辅助激光解吸电离飞行时间质谱法从这些蛋白质中成功鉴定出5种。为了进一步评估这5种差异蛋白，使用Panther程序将它们归类到适当的生物学过程。结论：大量已鉴定的蛋白质在细胞内信号传导 - 囊泡形成、结合、跨膜运输中发挥作用。这可能表明，在出现任何临床症状之前，肾脏糖尿病细胞损伤的最初迹象可能在尿液成分中显现出来。
