The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China.
State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China.
Int J Mol Sci. 2022 Mar 8;23(6):2920. doi: 10.3390/ijms23062920.
The ethylene-responsive element () is one of the keys and conserved transcription factors (TFs) in plants that play a vital role in regulating plant growth, development, and stress response. A total of 202 genes were identified from the pecan genome and renamed according to the chromosomal distribution of the genes. They were divided into four subfamilies according to the domain and phylogenetic analysis, including 26 , 168 , six , and two gene family members. These genes were distributed randomly across the 16 chromosomes, and we found 19 tandem and 146 segmental duplications which arose from ancient duplication events. The gene structure and conserved motif analysis demonstrated the conserved nature of intron/exon organization and motifs among the genes. Several cis-regulatory elements, which were related to light responsiveness, stress, and defense responses, were identified in the promoter regions of s. The expression profiling of 202 genes was assessed by using RNA-Seq data and qRT-PCR during development (pistillate flowering development, graft union development, and kernel development) and under abiotic stresses (waterlogging, drought). Moreover, the results suggested that the ERF-VII members may play a critical role in waterlogging stress. These findings provided new insights into gene evolution and divergence in pecan and can be considered a valuable resource for further functional validation, as well as for utilization in a stress-resistance-variety development program.
乙烯响应元件 () 是植物中关键的和保守的转录因子 (TF) 之一，在调节植物生长、发育和应激反应中起着至关重要的作用。从山核桃基因组中鉴定出 202 个基因，并根据基因的染色体分布重新命名。根据结构域和系统发育分析，它们分为四个亚家族，包括 26 个 、168 个 、六个 和两个 基因家族成员。这些基因随机分布在 16 条染色体上，我们发现了 19 个串联和 146 个片段重复，这些重复是由古老的复制事件产生的。基因结构和保守基序分析表明， 基因的内含子/外显子组织和基序在进化上是保守的。在启动子区域鉴定到了与光反应、应激和防御反应相关的多个顺式调控元件。使用 RNA-Seq 数据和 qRT-PCR 评估了 202 个基因在发育过程（雌花开花发育、嫁接结合发育和核仁发育）和非生物胁迫（淹水、干旱）下的表达谱。此外，结果表明 ERF-VII 成员可能在淹水胁迫中发挥关键作用。这些发现为山核桃中 基因的进化和分化提供了新的见解，可以作为进一步功能验证的有价值资源，也可以用于抗逆性品种开发计划。
