Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém 66075-110, PA, Brazil.
Graduate Program in Health and Society, University of the State of Rio Grande do Norte (UERN), Mossoró 59610-210, RN, Brazil.
Int J Mol Sci. 2022 Mar 16;23(6):3179. doi: 10.3390/ijms23063179.
Stroke is one of the leading causes of death and long-term disabilities worldwide, resulting in a debilitating condition occasioned by disturbances in the cerebral vasculature. Primary damage due to metabolic collapse is a quick outcome following stroke, but a multitude of secondary events, including excitotoxicity, inflammatory response, and oxidative stress cause further cell death and functional impairment. In the present work, we investigated whether a primary ischemic damage into the dorsal striatum may cause secondary damage in the circumjacent corpus callosum (CC). Animals were injected with endothelin-1 and perfused at 3, 7, 14, and 30 post-lesion days (PLD). Sections were stained with Cresyl violet for basic histopathology and immunolabeled by antibodies against astrocytes (anti-GFAP), macrophages/microglia (anti-IBA1/anti MHC-II), oligodendrocytes (anti-TAU) and myelin (anti-MBP), and Anti-Nogo. There were conspicuous microgliosis and astrocytosis in the CC, followed by later oligodendrocyte death and myelin impairment. Our results suggest that secondary white matter damage in the CC follows a primary focal striatal ischemia in adult rats.
中风是全球范围内导致死亡和长期残疾的主要原因之一，会导致大脑血管紊乱引起的身体虚弱状况。代谢崩溃导致的原发性损伤是中风后的快速结果，但多种继发性事件，包括兴奋毒性、炎症反应和氧化应激，会导致进一步的细胞死亡和功能障碍。在本工作中，我们研究了背侧纹状体的原发性缺血损伤是否会导致周围胼胝体（CC）的继发性损伤。动物在注射内皮素-1后，在 3、7、14 和 30 天（PLD）进行灌注。用 Cresyl 紫进行基本组织病理学染色，并使用针对星形胶质细胞（抗 GFAP）、巨噬细胞/小胶质细胞（抗 IBA1/抗 MHC-II）、少突胶质细胞（抗 TAU）和髓鞘（抗 MBP）以及抗 Nogo 的抗体进行免疫标记。在 CC 中出现明显的小胶质细胞增生和星形胶质细胞增生，随后出现少突胶质细胞死亡和髓鞘损伤。我们的结果表明，成年大鼠的背侧纹状体局灶性缺血后会继发 CC 白质损伤。
