一种利用2-硝基-5-苯乙酰氨基苯甲酸试纸筛选产青霉素G酰化酶细菌的方法。

A method for screening penicillin G acylase-producing bacteria by means of 2-nitro-5-phenylacetaminobenzoic acid test paper.

作者信息

Zhang Q J, Zhang L F, Han H X, Zhang Y Y

出版信息

Anal Biochem. 1986 Aug 1;156(2):413-6. doi: 10.1016/0003-2697(86)90274-5.

Abstract

A simple, rapid assay for screening penicillin G acylase-producing bacteria is presented. The method is based on the formation of yellow 2-nitro-5-aminobenzoic acid by penicillin G acylase acting on 2-nitro-5-phenylacetaminobenzoic acid (NIPAB). NIPAB test paper is briefly applied to bacterial colonies on the agar surface, which are subsequently scored individually on the paper by color; bright yellow indicates the presence of penicillin G acylase, natural color its absence. The present method is suitable not only for screening penicillin G acylase-production by a variety of bacteria but also for detection from a large number of transformant colonies of clones containing a gene encoding for the enzyme.

摘要

本文介绍了一种用于筛选产青霉素G酰化酶细菌的简单、快速检测方法。该方法基于青霉素G酰化酶作用于2-硝基-5-苯乙酰氨基苯甲酸（NIPAB）生成黄色的2-硝基-5-氨基苯甲酸。将NIPAB试纸短暂地贴在琼脂表面的细菌菌落上，随后根据试纸上菌落的颜色分别进行评分；亮黄色表明存在青霉素G酰化酶，自然颜色则表明不存在。本方法不仅适用于筛选多种细菌产生的青霉素G酰化酶，还适用于从大量含有该酶编码基因的克隆转化菌落中进行检测。