荧光胺与6-氨基青霉烷酸反应在青霉素酰化酶活性测定和检测中的应用。
Application of the fluorescamine reaction with 6-aminopenicillanic acid to estimation and detection of penicillin acylase activity.
作者信息
Baker W L
出版信息
Antimicrob Agents Chemother. 1983 Jan;23(1):26-30. doi: 10.1128/AAC.23.1.26.
Abstract
6-Aminopenicillanic acid may be quantitatively estimated by its reaction with fluorescamine in the concentration range of 1 to 10 micrograms/ml. The difference in reactivity between 6-aminopenicillanic acid and benzylpenicillin, which does not react with fluorescamine, can be used to determine penicillin acylase activity and obtain data on enzyme parameters and inhibitors. Unlike amino acids and peptides, 6-aminopenicillanic acid reacts strongly with fluorescamine at pH 4, an observation which can be used to determine the presence of penicillin acylase in whole bacterial cell preparations.
摘要
6-氨基青霉烷酸可通过其与荧光胺在1至10微克/毫升浓度范围内的反应进行定量测定。6-氨基青霉烷酸与不与荧光胺反应的苄青霉素之间的反应性差异，可用于测定青霉素酰化酶活性并获取有关酶参数和抑制剂的数据。与氨基酸和肽不同，6-氨基青霉烷酸在pH 4时与荧光胺强烈反应，这一观察结果可用于确定全细菌细胞制剂中青霉素酰化酶的存在。
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