Farkas Noémi-Izabella, Marincaș Laura, Barabás Réka, Bizo Liliana, Ilea Aranka, Turdean Graziella Liana, Toșa Monica, Cadar Oana, Barbu-Tudoran Lucian
Department of Chemical Engineering, Faculty of Chemistry and Chemical Engineering, Babeș-Bolyai University, 11 Arany János Street, RO-400028 Cluj-Napoca, Romania.
Department of Chemistry, Faculty of Chemistry and Chemical Engineering, Babeș-Bolyai University, 11 Arany János Street, RO-400028 Cluj-Napoca, Romania.
Materials (Basel). 2022 Mar 12;15(6):2105. doi: 10.3390/ma15062105.
The present study aimed to prepare nanofibers by electrospinning in the system polylactic acid-hydroxyapatite-doxycycline (PLA-HAP-Doxy) to be used as a drug delivery vehicle. Two different routes were employed for the preparation of Doxy-containing nanofibers: Immobilization on the electrospun mat's surface and encapsulation in the fiber structure. The nanofibers obtained by Doxy encapsulation were characterized using Fourier transform infrared (FTIR) spectroscopy, thermogravimetric (TG) and differential thermal analyses (DTA) and scanning electron microscopy (SEM). The adsorption properties of pure PLA and PLA-HAP nanofibers were investigated for solutions with different Doxy concentrations (3, 7 and 12 wt%). Moreover, the desorption properties of the active substance were tested in two different fluids, simulated body fluid (SBF) and phosphate buffer solution (PBS), to evidence the drug release properties. In vitro drug release studies were performed and different drug release kinetics were assessed to confirm the use of these nanofiber materials as efficient drug delivery vehicles. The obtained results indicate that the PLA-HAP-Doxy is a promising system for biomedical applications, the samples with 3 and 7 wt% of Doxy-loaded PLA-HAP nanofibers prepared by physical adsorption are the most acceptable membranes to provide prolonged release in PBS/SBF rather than an immediate release of Doxy.
本研究旨在通过静电纺丝在聚乳酸-羟基磷灰石-多西环素（PLA-HAP-Doxy）体系中制备纳米纤维，用作药物递送载体。制备含多西环素纳米纤维采用了两种不同途径：固定在静电纺丝垫表面和包封在纤维结构中。通过多西环素包封获得的纳米纤维使用傅里叶变换红外（FTIR）光谱、热重（TG）和差热分析（DTA）以及扫描电子显微镜（SEM）进行表征。研究了纯PLA和PLA-HAP纳米纤维对不同多西环素浓度（3、7和12 wt%）溶液的吸附性能。此外，在两种不同流体，即模拟体液（SBF）和磷酸盐缓冲溶液（PBS）中测试了活性物质的解吸性能，以证明药物释放特性。进行了体外药物释放研究并评估了不同的药物释放动力学，以确认这些纳米纤维材料作为高效药物递送载体的用途。所得结果表明，PLA-HAP-Doxy是一种有前景的生物医学应用体系，通过物理吸附制备的含3 wt%和7 wt%多西环素的PLA-HAP纳米纤维样品是最适合的膜，可在PBS/SBF中实现多西环素的缓释而非速释。
