Zafar Farjad, Sheraz Muhammad Ali, Ali Syed Abid, Riaz Maryam, Ahmed Sofia, Kazi Sadia Hafeez, Tariq Safoora, Anwar Zubair
Baqai Dental College, Baqai Medical University, Karachi, 75340, Pakistan.
Baqai Institute of Pharmaceutical Sciences, Baqai Medical University, Karachi, 75340, Pakistan.
Sci Rep. 2025 Jul 1;15(1):20519. doi: 10.1038/s41598-025-05894-1.
It is essential to adopt effective therapy strategies for periodontal diseases to achieve optimal results while avoiding adverse effects on the system. This study has developed various PEGylated chitosan-based biodegradable xerogels for localized release of doxycycline hyclate (DH) to treat periodontal infectious diseases. The xerogels were formulated using the solvent casting method, and the solvent (0.25 M HCl) was slowly evaporated at ambient conditions. Two different molecular weights were employed for chitosan and polyethylene glycol, and twelve combinations, including the placebos and controls, were prepared for the formulation of xerogels. Different physical and chemical characteristics of the prepared DH xerogels were studied, such as drying time and rate, thickness, moisture content, swelling index, organoleptic characteristics, scanning electron microscopy, FTIR spectrometry, differential scanning calorimetry, drug release and kinetics, and antibacterial activity. The results revealed that the drug transforms from a crystalline to an amorphous state, thus rapidly releasing the drug (> 60% in 30 min. in all xerogels), followed by a sustained release up to 10 h. The release kinetics results revealed that the drug followed the Korsmeyer-Peppas model. It is concluded that the formulated DH-loaded xerogels showed promising results for use in the periodontal pockets to treat various infectious diseases.
为了在避免对全身产生不良反应的同时获得最佳疗效，采用有效的牙周疾病治疗策略至关重要。本研究开发了多种基于聚乙二醇化壳聚糖的可生物降解干凝胶，用于局部释放盐酸多西环素（DH）以治疗牙周感染性疾病。干凝胶采用溶剂浇铸法制备，溶剂（0.25M盐酸）在环境条件下缓慢蒸发。壳聚糖和聚乙二醇采用两种不同的分子量，制备了包括安慰剂和对照在内的十二种组合用于干凝胶的配方。研究了制备的DH干凝胶的不同物理和化学特性，如干燥时间和速率、厚度、水分含量、溶胀指数、感官特性、扫描电子显微镜、傅里叶变换红外光谱、差示扫描量热法、药物释放和动力学以及抗菌活性。结果表明，药物从结晶态转变为非晶态，从而迅速释放药物（所有干凝胶在30分钟内释放>60%），随后持续释放长达10小时。释放动力学结果表明，药物遵循Korsmeyer-Peppas模型。得出的结论是，所制备的载有DH的干凝胶在用于牙周袋治疗各种感染性疾病方面显示出有前景的结果。
