BS5次生代谢产物的分离、鉴定及杀虫活性

Yue Ying, Jiang Mingfang, Hu Hanying, Wu Jinghui, Sun Haoran, Jin Hong, Hou Taiping, Tao Ke

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610017, China.

J Fungi (Basel). 2022 Mar 11;8(3):288. doi: 10.3390/jof8030288.

The fungal strain BS5 was isolated from a soil sample collected in the Tibetan Plateau, which displayed good insecticidal activity and was identified as based on morphological and molecular analysis. This study aimed to evaluate the insecticidal activity and identify the active compound of the strain BS5 against the locust . The insecticidal activity of the fermented broth of BS5 was at 100% after 7 days against locusts. We extracted the fermented broth of BS5 and then evaluated the insecticidal activity of the extracts against locusts. The ethyl acetate extract exhibited promising activity levels with an LC value of 1077.94 μg/mL and was separated through silica gel column chromatography. The UPLC-Q-Exactive Orbitrap/MS system was employed to analyze the active fraction Fr2.2.2 (with an LC value of 674.87 μg/mL), and two compounds were identified: phellamurin and rubratoxin B.

真菌菌株BS5是从青藏高原采集的土壤样本中分离得到的，它表现出良好的杀虫活性，并通过形态学和分子分析进行了鉴定。本研究旨在评估菌株BS5对蝗虫的杀虫活性并鉴定其活性化合物。BS5发酵液在7天后对蝗虫的杀虫活性达到100%。我们提取了BS5的发酵液，然后评估提取物对蝗虫的杀虫活性。乙酸乙酯提取物表现出良好的活性水平，LC值为1077.94μg/mL，并通过硅胶柱色谱进行分离。采用超高效液相色谱-四极杆-静电场轨道阱质谱系统分析活性馏分Fr2.2.2（LC值为674.87μg/mL），鉴定出两种化合物：桑辛素和红天精毒素B。