Department of Microbiology & Fermentation Technology, CSIR-Central Food Technological Research Institute, Mysuru 570 020, Karnataka, India; Academy of Scientific and Innovative Research, CSIR-Central Food Technological Research Institute, Mysuru 570 020, Karnataka, India.
Grain Science & Technology, CSIR-Central Food Technological Research Institute, Mysuru 570 020, Karnataka, India.
Food Chem. 2018 Jul 30;255:89-96. doi: 10.1016/j.foodchem.2018.02.034. Epub 2018 Feb 8.
An account of antioxidant and antimicrobial characteristics displayed by ethyl acetate extract of Talaromyces purpureogenus CFRM-02 cultivated by submerged fermentation on bengal gram husk (BegH) led to the isolation of a new molecule, which was purified via silica gel column chromatography. The TLC and HPLC data revealed the purity of the isolated compound. By employing an array of spectroscopic techniques, structural elucidation was accomplished, and the molecule was identified as 2-hydroxymethyl-benzoic acid 2'-hydroxy-tetradecyl ester and was also designated with a familiar name pentalsamonin. The pentalsamonin molecule scavenged DPPH (24%), HO (64%), superoxide anion (58%) with a reducing power (0.989 AU) at the given concentration. The MIC and MBC of pentalsamonin against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae are in the range 62.5-125 and 125-250 µg/ml, respectively. The antioxidant and antibacterial functional attributes of pentalsamonin suggest useful applications in food and nutraceuticals.
紫红红曲霉 CFRM-02 经摇瓶发酵在豇豆壳上培养，其乙酸乙酯提取物具有抗氧化和抗菌特性，由此分离得到一种新分子，该分子经硅胶柱层析法进行了纯化。TLC 和 HPLC 数据显示分离化合物的纯度。采用一系列光谱技术完成了结构解析，鉴定该分子为 2-羟甲基苯甲酸 2'-羟基十四烷基酯，并赋予其一个熟知的名称 pentalsamonin。该 pentalsamonin 分子对 DPPH（24%）、HO（64%）、超氧阴离子（58%）具有清除作用，在给定浓度下的还原能力为 0.989 AU。pentalsamonin 对枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌和肺炎克雷伯菌的 MIC 和 MBC 分别在 62.5-125 和 125-250 μg/ml 范围内。pentalsamonin 的抗氧化和抗菌功能特性表明其在食品和营养保健品中有潜在的应用价值。
