Corcobado Tamara, Milenković Ivan, Saiz-Fernández Iñigo, Kudláček Tomáš, Plichta Roman, Májek Tomáš, Bačová Aneta, Ďatková Henrieta, Dálya László Benedek, Trifković Miloš, Mureddu Davide, Račko Vladimír, Kardošová Monika, Ďurkovič Jaroslav, Rattunde Roman, Jung Thomas
Phytophthora Research Centre, Faculty of Forestry and Wood Technology, Mendel University in Brno, 61300 Brno, Czech Republic.
Faculty of Forestry, University of Belgrade, Kneza Višeslava 1, 11030 Belgrade, Serbia.
J Fungi (Basel). 2022 Mar 14;8(3):298. doi: 10.3390/jof8030298.
infections are followed by histological alterations, physiological and metabolomic adjustments in the host but very few studies contemplate these changes simultaneously. seedlings were inoculated with A1 and A2 mating types of the heterothallic and with the homothallic to identify plant physiological and metabolomic changes accompanying microscope observations of the colonization process one, two and three weeks after inoculation. -infected plants died at a faster pace than those inoculated with and showed higher mortality than A1-infected plants. A1 and A2 caused similar progression and total rate of mortality. Most differences in the physiological parameters between inoculated and non-inoculated plants were detected two weeks after inoculation. Alterations in primary and secondary metabolites in roots and leaves were demonstrated for all the inoculated plants two and three weeks after inoculation. The results indicate that is more aggressive to seedlings than both mating types of while A1 showed a slower infection mode than A2 and led to minor plant metabolomic adjustments.
感染后，宿主会出现组织学改变、生理和代谢组学调整，但很少有研究同时考虑这些变化。用异宗配合的A1和A2交配型以及同宗配合的菌株接种幼苗，以确定接种后1周、2周和3周在显微镜下观察到的定殖过程中伴随的植物生理和代谢组学变化。感染的植物比接种的植物死亡速度更快，且死亡率高于A1感染的植物。A1和A2导致的死亡进程和总死亡率相似。接种和未接种植物之间生理参数的大多数差异在接种后2周被检测到。接种后2周和3周，所有接种植物的根和叶中的初级和次级代谢产物都发生了变化。结果表明，与两种交配型的相比，对幼苗更具侵袭性，而A1的感染模式比A2慢，导致植物代谢组学调整较小。
