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脐带间充质干细胞联合表面活性蛋白 B 通过调控巨噬细胞极化缓解急性呼吸窘迫综合征的炎症反应。

Umbilical Cord-derived Mesenchymal Stem Cells with Surfactant Protein B Alleviates Inflammatory Response in Acute Respiratory Distress Syndrome by Regulating Macrophage Polarization.

机构信息

Department of Pediatrics, General Hospital of Central Theater Command of Chinese People's Liberation Army, Wuhan, China.

出版信息

Balkan Med J. 2022 Mar 14;39(1):130-139. doi: 10.4274/balkanmedj.galenos.2021.2021-9-8.

DOI:10.4274/balkanmedj.galenos.2021.2021-9-8
PMID:35330560
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8941227/
Abstract

BACKGROUND

Acute respiratory distress syndrome (ARDS) is a severe disorder that is related to a high mortality. Mesenchymal stem cells (MSCs) have shown strong effects in relieving lung injury.

AIMS

To determine the role of umbilical cord-derived MSCs (UC-MSCs) together with surfactant protein B (SP-B) in ARDS.

STUDY DESIGN

Animal experimentation.

METHODS

Immunophenotypic characteristics of UC-MSCs were identified. BALB/c mice were intratracheally administrated with lipopolysaccharide (LPS) and received UC-MSCs or UC-MSCs transfected with SP-B (UC-MSCs-SP-B). Pathological changes and lung injury degrees after transplantation were assessed by histological and biochemical analyses. Inflammatory chemokine and cytokine production in the bronchoalveolar lavage fluid (BALF) was measured using enzyme-linked immunoassay. Flow cytometry was used to examine macrophage phenotypes and differentiation of T-helper 17 (Th17) and T-regulatory (Treg) in the BALF.

RESULTS

Our results showed that isolated UC-MSCs possessed multilineage differentiation potential. SP-B transfection into UC-MSCs strengthened the effects of UC-MSCs on lung function repair in LPS-induced ARDS. UC-MSCs and UC-MSCs-SP-B attenuated cellular infiltration. Additionally, UC-MSCs and UC-MSCs-SP-B inhibited the inflammatory response by promoting M2-like polarization, as well as reduced Th17 differentiation and promoted Treg differentiation.

CONCLUSION

UC-MSCs in combination with SP-B, alleviates inflammatory reaction in ARDS by regulating macrophage polarization.

摘要

背景

急性呼吸窘迫综合征（ARDS）是一种严重的疾病，死亡率较高。间充质干细胞（MSCs）在缓解肺损伤方面显示出强大的作用。

目的

确定脐带间充质干细胞（UC-MSCs）与表面活性蛋白 B（SP-B）联合在 ARDS 中的作用。

研究设计

动物实验。

方法

鉴定 UC-MSCs 的免疫表型特征。用脂多糖（LPS）气管内滴注 BALB/c 小鼠，并给予 UC-MSCs 或转染 SP-B 的 UC-MSCs（UC-MSCs-SP-B）。通过组织学和生化分析评估移植后病理变化和肺损伤程度。酶联免疫吸附试验测定支气管肺泡灌洗液（BALF）中炎症趋化因子和细胞因子的产生。流式细胞术检测 BALF 中巨噬细胞表型和 T 辅助 17（Th17）和 T 调节（Treg）的分化。

结果

我们的结果表明，分离的 UC-MSCs 具有多能分化潜能。SP-B 转染到 UC-MSCs 中增强了 UC-MSCs 在 LPS 诱导的 ARDS 中对肺功能修复的作用。UC-MSCs 和 UC-MSCs-SP-B 减轻了细胞浸润。此外，UC-MSCs 和 UC-MSCs-SP-B 通过促进 M2 样极化来抑制炎症反应，减少 Th17 分化，促进 Treg 分化。

结论

UC-MSCs 与 SP-B 联合应用通过调节巨噬细胞极化减轻 ARDS 中的炎症反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d6f/8941227/c4960bca1f6e/BMJ-39-130-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d6f/8941227/4800f404ea65/BMJ-39-130-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d6f/8941227/535a3a58a608/BMJ-39-130-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d6f/8941227/5fba17c21dd1/BMJ-39-130-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d6f/8941227/15906f525db1/BMJ-39-130-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d6f/8941227/c4960bca1f6e/BMJ-39-130-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d6f/8941227/4800f404ea65/BMJ-39-130-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d6f/8941227/535a3a58a608/BMJ-39-130-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d6f/8941227/5fba17c21dd1/BMJ-39-130-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d6f/8941227/15906f525db1/BMJ-39-130-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d6f/8941227/c4960bca1f6e/BMJ-39-130-g5.jpg

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