比较毛细支气管炎患儿与呼吸道合胞病毒引起和非引起毛细支气管炎患儿的粪便微生物群分析。

Comparative Fecal Microbiota Analysis of Infants With Acute Bronchiolitis Caused or Not Caused by Respiratory Syncytial Virus.

机构信息

Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy.

Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy.

出版信息

Front Cell Infect Microbiol. 2022 Mar 7;12:815715. doi: 10.3389/fcimb.2022.815715. eCollection 2022.

DOI:10.3389/fcimb.2022.815715

PMID:35330643

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8940166/

Abstract

Bronchiolitis due to respiratory syncytial virus (RSV) or non-RSV agents is a health-menacing lower respiratory tract (LRT) disease of infants. Whereas RSV causes more severe disease than other viral agents may, genus-dominant fecal microbiota profiles have been identified in US hospitalized infants with bronchiolitis. We investigated the fecal microbiota composition of infants admitted to an Italian hospital with acute RSV (25/37 [67.6%]; group I) or non-RSV (12/37 [32.4%]; group II) bronchiolitis, and the relationship of fecal microbiota characteristics with the clinical characteristics of infants. Group I and group II infants differed significantly (24/25 [96.0%] versus 5/12 [41.7%]; = 0.001) regarding 90% oxygen saturation (SpO), which is an increased respiratory effort hallmark. Accordingly, impaired feeding in infants from group I was significantly more frequent than in infants from group II (19/25 [76.0%] versus 4/12 [33.3%]; = 0.04). Conversely, the median (IQR) length of stay was not significantly different between the two groups (seven [3-14] for group I versus five [5-10] for group II; = 0.11). The 16S ribosomal RNA V3-V4 region amplification of infants' fecal samples resulted in 299 annotated amplicon sequence variants. Based on alpha- and beta-diversity microbiota downstream analyses, group I and group II infants had similar bacterial communities in their samples. Additionally, comparing infants having <90% SpO ( = 29) with infants having ≥90% SpO ( = 8) showed that well-known dominant genera (, , /, and /) were differently, but not significantly ( = 0.44, = 0.71, = 0.98, and = 0.41, respectively) abundant between the two subgroups. Overall, we showed that, regardless of RSV or non-RSV bronchiolitis etiology, no fecal microbiota-composing bacteria could be associated with the severity of acute bronchiolitis in infants. Larger and longitudinally conducted studies will be necessary to confirm these findings.

摘要

呼吸道合胞病毒（RSV）或非 RSV 病原体引起的细支气管炎是婴儿下呼吸道（LRT）的一种威胁健康的疾病。虽然 RSV 引起的疾病比其他病毒病原体更严重，但在美国住院的细支气管炎婴儿中，已确定了以属为主的粪便微生物群特征。我们调查了因急性 RSV（25/37 [67.6%]；I 组）或非 RSV（12/37 [32.4%]；II 组）细支气管炎住院的意大利婴儿的粪便微生物群组成，以及粪便微生物群特征与婴儿临床特征的关系。I 组和 II 组婴儿在 90%氧饱和度（SpO）方面存在显著差异（24/25 [96.0%]与 5/12 [41.7%]；= 0.001），这是呼吸努力增加的标志。相应地，I 组婴儿的喂养受损明显比 II 组婴儿更频繁（19/25 [76.0%]与 4/12 [33.3%]；= 0.04）。相反，两组之间的中位（IQR）住院时间无显著差异（I 组为 7 [3-14]，II 组为 5 [5-10]；= 0.11）。婴儿粪便样本的 16S 核糖体 RNA V3-V4 区域扩增产生了 299 个注释的扩增子序列变体。基于 alpha 和 beta 多样性微生物组下游分析，I 组和 II 组婴儿的样本中具有相似的细菌群落。此外，将 SpO ＜90%的婴儿（= 29）与 SpO ≥90%的婴儿（= 8）进行比较，结果表明，已知的优势属（、、/和/）在这两个亚组之间的丰度不同，但无统计学意义（= 0.44，= 0.71，= 0.98，= 0.41，分别）。总体而言，我们表明，无论 RSV 或非 RSV 细支气管炎的病因如何，急性细支气管炎婴儿粪便微生物群组成细菌与疾病的严重程度无关。需要更大规模和纵向进行的研究来证实这些发现。