呼吸道合胞病毒细支气管炎中的氧化应激与炎性血浆生物标志物

Oxidative stress and inflamatory plasma biomarkers in respiratory syncytial virus bronchiolitis.

作者信息

Moreno-Solís Gloria, Dela Torre-Aguilar María José, Torres-Borrego Javier, Llorente-Cantarero Francisco Jesus, Fernández-Gutiérrez Fernando, Gil-Campos Mercedes, Túnez-Fiñana Isaac, Pérez-Navero Juan Luis

机构信息

Department of Paediatrics, Paediatric Pulmonology and Allergy Unit, Reina Sofia University Hospital, Cordoba, Spain.

Faculty of Medicine, Maimonides Institute for Biomedical Research of Cordoba (IMIBIC), University of Cordoba, Spain.

出版信息

Clin Respir J. 2017 Nov;11(6):839-846. doi: 10.1111/crj.12425. Epub 2016 Jan 6.

DOI:10.1111/crj.12425

PMID:26663823

Abstract

INTRODUCTION

Oxidative stress (OS) plays a crucial role in the pathogenesis of inflammatory lung diseases.

OBJECTIVES

(i) We determined whether acute bronchiolitis (AB) caused by respiratory syncytial virus (RSV) induced OS; (ii) assessed whether OS biomarkers correlated with the severity of RSV-AB; and (iii) studied whether the levels of interleukins are associated with OS biomarkers.

METHODS

We performed an observational study by comparing healthy infants (Group 1) with RSV-AB infants, classified as Group 2 (pulse oximetry (SpO ) >93%), and Group 3 (SpO ≤ 92%), which needed oxygen therapy. Blood samples were collected to determine the levels of lipid peroxidation (LPO) products (LPO), total glutathione (TG), oxidised glutathione (GSSG), reduced glutathione (GSH), glutathione peroxidase (GPx), interleukins (ILs) IL-10, IL-6, IL-8, interferon-gamma (IFNγ), tumour necrosis factor-alpha (TNFα) and macrophage inflammatory proteins (MIP α and MIP β).

RESULTS

Forty-six RSV-AB infants (47% needed oxygen therapy) and 27 healthy infants were included. The GSH/GSSG ratio was lower in RSV-AB infants than in Group 1 (P<0.001). GSSG and GPx were significantly higher in Group 3. GSSG predicted the need for oxygen therapy with an optimal cut-off point of 15 µM/g for haemoglobin. The GSH/GSSG ratio negatively correlated with IL-6 (P: 0.014), IL-8 (P: 0.014) and IL-10 (P: 0.033). Group 3 exhibited a direct correlation between GPx and IL-10 levels (P: 0.024) and between LPO and MIP β (P: 0.003).

CONCLUSIONS

RSV induced OS in AB. An increase in GSSG correlated with the disease severity in the infants. OS may contribute to the pathogenesis of RSV-AB.

摘要

引言

氧化应激（OS）在炎症性肺部疾病的发病机制中起关键作用。

目的

（i）我们确定呼吸道合胞病毒（RSV）引起的急性细支气管炎（AB）是否诱导了OS；（ii）评估OS生物标志物是否与RSV-AB的严重程度相关；以及（iii）研究白细胞介素水平是否与OS生物标志物相关。

方法

我们通过比较健康婴儿（第1组）与RSV-AB婴儿进行了一项观察性研究，RSV-AB婴儿分为第2组（脉搏血氧饱和度（SpO）>93%）和第3组（SpO≤92%），第3组需要氧疗。采集血样以测定脂质过氧化（LPO）产物（LPO）、总谷胱甘肽（TG）、氧化型谷胱甘肽（GSSG）、还原型谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPx）、白细胞介素（ILs）IL-10、IL-6、IL-8、干扰素-γ（IFNγ）、肿瘤坏死因子-α（TNFα）和巨噬细胞炎性蛋白（MIPα和MIPβ）的水平。

结果

纳入了46例RSV-AB婴儿（47%需要氧疗）和27例健康婴儿。RSV-AB婴儿的GSH/GSSG比值低于第1组（P<0.001）。第3组的GSSG和GPx显著更高。GSSG预测氧疗需求的最佳血红蛋白截断点为15µM/g。GSH/GSSG比值与IL-6（P：0.014）、IL-8（P：0.014）和IL-10（P：0.033）呈负相关。第3组显示GPx与IL-10水平之间存在直接相关性（P：0.024），LPO与MIPβ之间存在直接相关性（P：0.003）。