Giannattasio-Ferraz Silvia, Ene Adriana, Gomes Vitor Júnio, Queiroz Cid Oliveira, Maskeri Laura, Oliveira André Penido, Putonti Catherine, Barbosa-Stancioli Edel F
Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Bioinformatics Program, Loyola University Chicago, Chicago, IL, United States.
Front Microbiol. 2022 Mar 7;13:764760. doi: 10.3389/fmicb.2022.764760. eCollection 2022.
The study of livestock microbiota has immediate benefits for animal health as well as mitigating food contamination and emerging pathogens. While prior research has indicated the gastrointestinal tract of cattle as the source for many zoonoses, including Shiga-toxin producing and antibiotic resistant bacteria, the bovine urinary tract microbiota has yet to be thoroughly investigated. Here, we describe 5 and 4 strains isolated from urine of dairy Gyr cattle. While both species are typically associated with urinary tract infections and mastitis, all of the animals sampled were healthy. The bovine urinary strains were compared to and isolates from other bovine samples as well as human urinary samples. While the bovine urinary isolates had genomic similarity to isolates from the gastrointestinal tract of cattle and other agricultural animals, the bovine urinary strains were most similar to human isolates suggesting niche adaptation rather than host adaptation. Examination of prophages harbored by these bovine isolates revealed similarity with prophages within distantly related and isolates from the human urinary tract. This suggests that related urinary phages may persist and/or be shared between mammals. Future studies of the bovine urinary microbiota are needed to ascertain if and are resident members of this niche and/or possible sources for emerging pathogens in humans.
对家畜微生物群的研究对动物健康以及减轻食物污染和新出现的病原体具有直接益处。虽然先前的研究表明牛的胃肠道是包括产志贺毒素菌和抗生素耐药菌在内的许多人畜共患病的来源，但牛的泌尿道微生物群尚未得到充分研究。在此，我们描述了从吉尔奶牛尿液中分离出的5株和4株菌株。虽然这两个菌种通常与尿路感染和乳腺炎有关，但所有采样的动物都是健康的。将牛的泌尿道菌株与从其他牛样本以及人类尿液样本中分离出的菌株进行了比较。虽然牛的泌尿道分离株与从牛和其他家畜胃肠道分离出的菌株具有基因组相似性，但牛的泌尿道菌株与人类分离株最为相似，这表明是生态位适应而非宿主适应。对这些牛分离株携带的原噬菌体的检查发现，它们与来自人类泌尿道的远缘相关分离株中的原噬菌体具有相似性。这表明相关的泌尿道噬菌体可能在哺乳动物之间持续存在和/或共享。需要对牛的泌尿道微生物群进行进一步研究，以确定这些菌株是否是该生态位的常驻成员和/或人类新出现病原体的可能来源。
