Reynolds C A, Scuderi R A, Skidmore A L, Duniere L, Morrison S Y
William H. Miner Agricultural Research Institute, Chazy, New York, USA.
Lallemand Specialties Inc., Milwaukee, Wisconsin, USA.
Microbiol Spectr. 2025 Jul;13(7):e0330224. doi: 10.1128/spectrum.03302-24. Epub 2025 May 28.
Application of a beneficial microbial cocktail of spp. and spp. was evaluated first for adherence to polyethylene calf hutch material, and second, to determine if application to individual calf hutches post-cleaning influenced surface recolonization by enteric pathogens. Three treatments were utilized: (i) no application (), (ii) chlorine-free, distilled water (), or (iii) an application of a microbial inoculant containing spp. and spp. at a concentration of 0.4 g/m of hutch space (). Thirty-six 15 × 15 cm pieces of naïve, sterile polyethylene calf hutch material received either NC or LF and were incubated at 28°C, and bacterial growth was evaluated by total aerobic plate counts at 24, 48, and 72 h post-application. Thirty polyethylene calf hutches ( = 10/treatment) were randomized to NC, DW, or LF 24 h after cleaning. Calves were placed in the hutches 24 h after treatment application and monitored daily for 28 d. surface samples were randomized by time from five unique locations within the calf hutch interior: 24 h post-cleaning and then 24 h, 7 d, 14 d, and 21 d post-application. Total aerobic plate counts and culture-independent approaches RT-qPCR and 16S amplicon sequencing were used to detect and identify the composition of the bacterial community . The bacteria in the inoculant were able to successfully colonize on polyethylene, and application to individual polyethylene calf housing influenced microbial diversity and reduced the presence of some undesirable bacteria on high-contact interior surfaces.IMPORTANCEDue to its multifactorial nature, neonatal calf diarrhea can be difficult to manage on farms. Clean housing environments are a critical disease control point, especially for calves less than one month of age. Application of a beneficial biofilm-forming bacterial product after cleaning of neonatal calf housing may influence the microbial communities present on the surface, particularly those that may present disease risk to calves in early life.
首先评估了 spp. 和 spp. 的有益微生物组合对聚乙烯犊牛栏材料的附着情况，其次，确定清洁后在单个犊牛栏中应用该组合是否会影响肠道病原体的表面再定殖。采用了三种处理方法：(i) 不应用（），(ii) 无氯蒸馏水（），或 (iii) 以 0.4 g/m 的栏舍空间浓度应用含有 spp. 和 spp. 的微生物接种剂（）。36 块 15×15 cm 的未使用过的无菌聚乙烯犊牛栏材料接受 NC 或 LF 处理，并在 28°C 下孵育，在应用后 24、48 和 72 小时通过总需氧平板计数评估细菌生长。30 个聚乙烯犊牛栏（每组 10 个）在清洁后 24 小时随机分为 NC、DW 或 LF 组。在处理应用后 24 小时将犊牛放入栏中，并每天监测 28 天。从犊牛栏内部五个不同位置按时间随机采集表面样本：清洁后 24 小时，然后在应用后 24 小时、7 天、14 天和 21 天。使用总需氧平板计数和非培养方法 RT-qPCR 和 16S 扩增子测序来检测和鉴定细菌群落的组成。接种剂中的细菌能够成功定殖在聚乙烯上，应用于单个聚乙烯犊牛栏会影响微生物多样性，并减少高接触内表面上一些不良细菌的存在。
重要性
由于其多因素性质，新生犊牛腹泻在农场中可能难以管理。清洁的饲养环境是关键的疾病控制点，特别是对于小于 1 个月龄的犊牛。在清洁新生犊牛栏后应用有益的生物膜形成细菌产品可能会影响表面存在的微生物群落，特别是那些可能在犊牛早期生命中带来疾病风险的群落。
