Unidad de Investigación Médica en Bioquímica. Hospital de Especialidades. Centro Médico Nacional Siglo XXI. Instituto Mexicano del Seguro Social. Mexico City, Mexico; Laboratorio de Biotecnología y Bioinformática Genómica, Departamento de Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico.
Unidad de Investigación en Epidemiología Clínica de la UMAE, Hospital de Especialidades, Centro Médico Nacional Siglo XXI. Instituto Mexicano del Seguro Social. Mexico City, Mexico.
J Nutr Biochem. 2022 Jul;105:108996. doi: 10.1016/j.jnutbio.2022.108996. Epub 2022 Mar 21.
While several studies have previously described the levels of one-carbon metabolism-related micronutrients in women with gestational diabetes mellitus (GDM) and their neonates, the results in these literature reports have been contradictory. We hypothesized that the concentrations of micronutrients involved in the one-carbon cycle are altered in pregnant women and their neonates by GDM, and that these changes could further modify the neonatal anthropometry. Micronutrient levels were measured in 123 pregnant women with normal glucose levels (M-ND) and their neonates (N-ND), as well as in 54 pregnant women with gestational diabetes (M-GDM) and their neonates (M-GDM). Folate and vitamin B12 levels were measured via competitive ELISA, and betaine, choline, and glycine levels were measured via ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS/MS). Vitamin B12 and Glycine were found to be higher in M-GDM compared to M-ND. N-GDM had higher levels of folic acid and vitamin B12 and lower levels of betaine and choline compared to N-ND. In general, neonates presented with high concentrations of micronutrients compared to their mothers, and the fetus/maternal ratio of micronutrients was higher among the N-ND as compared to the N-GDM. Micronutrients were also variably associated with anthropometric measurements. The association of betaine with neonatal anthropometry in N-GDM is highlighted. In summary, our results implicate a potential role of GDM in altering the levels of one-carbon metabolism-related micronutrients among pregnant women and their neonates. Likewise, our results also elucidate a potential association between the concentrations of micronutrients and the weight, height, and head circumference of neonates.
虽然之前有几项研究描述了妊娠糖尿病（GDM）女性及其新生儿的一碳代谢相关微量营养素水平，但这些文献报道的结果存在矛盾。我们假设 GDM 改变了孕妇及其新生儿体内参与一碳循环的微量营养素的浓度，这些变化可能进一步改变新生儿的人体测量学指标。在 123 名血糖正常的孕妇及其新生儿（M-ND）以及 54 名妊娠糖尿病孕妇及其新生儿（M-GDM）中测量了微量营养素水平。叶酸和维生素 B12 水平通过竞争性 ELISA 测定，甜菜碱、胆碱和甘氨酸水平通过超高效液相色谱-质谱联用（UHPLC-MS/MS）测定。与 M-ND 相比，M-GDM 中维生素 B12 和甘氨酸的含量较高。与 N-ND 相比，N-GDM 的叶酸和维生素 B12 水平较高，甜菜碱和胆碱水平较低。一般来说，与母亲相比，新生儿的微量营养素浓度较高，与 N-GDM 相比，N-ND 的胎儿/母亲微量营养素比值较高。微量营养素与人体测量学指标也存在不同程度的关联。强调了甜菜碱与 N-GDM 新生儿人体测量学的关联。总之，我们的研究结果表明，GDM 可能在改变孕妇及其新生儿一碳代谢相关微量营养素水平方面发挥作用。同样，我们的研究结果也阐明了微量营养素浓度与新生儿体重、身高和头围之间的潜在关联。
