Belarbi Aicha Yasmine, de Almeida Otávio G G, Gatto Veronica, Torriani Sandra, Del Rio Beatriz, Ladero Victor, Redruello Begoña, Bensalah Farid, Alvarez Miguel A
Microbial Genetics Laboratory, Department of Biology, Faculty of Nature and Life Sciences, University Ahmed Ben Bella, Oran1-Oran, 31000, Es Senia, Algeria.
Department of Clinical Analysis, Toxicology and Food Science, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida Do Café, s/n, Ribeirão Preto, SP, 14040-903, Brazil.
Arch Microbiol. 2022 Mar 25;204(4):220. doi: 10.1007/s00203-022-02828-7.
Currently, consumption of spontaneously fermented milks is common in Algeria, making it a feasible source of diverse lactic acid bacteria (LAB) with the potential to be used as adjunct cultures to improve quality and safety of fermented dairy products. In this context, to select eligible indigenous strains which could be applied as bioprotective and/or starter cultures, the present study aimed to characterize the genomic variability, biotechnological potential, and safety of thirty-eight LAB isolated from Algerian dairy and farm sources of western Algeria. The isolates were unequivocally identified by 16S rRNA gene and fingerprint-based methods. The following species were identified: Enterococcus faecium (n = 15), Enterococcus durans (n = 2), Enterococcus hirae (n = 2), Enterococcus lactis (n = 1), Lactiplantibacillus plantarum (n = 6), Lactococcus lactis (n = 4), Levilactobacillus brevis (n = 3), Lacticaseibacillus paracasei (n = 3), Lacticaseibacillus rhamnosus (n = 1), and Pediococcus acidilactici (n = 1). Among the strains, three of them, L. lactis LGMY8, Lb. plantarum LGMY30 and Lb. paracasei LGMY31 were safe and showed some valuable biotechnological properties, such as high acidification, proteolytic activity, EPS production, and inhibition of undesirable bacteria that made them powerful candidates to be used as starter.
目前，自发发酵乳在阿尔及利亚很常见，这使其成为多种乳酸菌（LAB）的可行来源，这些乳酸菌有潜力用作辅助培养物，以提高发酵乳制品的质量和安全性。在此背景下，为了筛选出可作为生物保护剂和/或发酵剂培养物应用的合格本土菌株，本研究旨在对从阿尔及利亚西部乳制品和农场来源分离出的38株乳酸菌的基因组变异性、生物技术潜力和安全性进行表征。通过16S rRNA基因和基于指纹图谱的方法明确鉴定了这些分离株。鉴定出的菌株如下：粪肠球菌（n = 15）、耐久肠球菌（n = 2）、海氏肠球菌（n = 2）、乳酸肠球菌（n = 1）、植物乳杆菌（n = 6）、乳酸乳球菌（n = 4）、短乳杆菌（n = 3）、副干酪乳杆菌（n = 3）、鼠李糖乳杆菌（n = 1）和嗜酸乳杆菌（n = 1）。在这些菌株中，乳酸乳球菌LGMY8、植物乳杆菌LGMY30和副干酪乳杆菌LGMY31这三株菌是安全的，并表现出一些有价值的生物技术特性，如高酸化能力、蛋白水解活性、胞外多糖产生以及对有害细菌的抑制作用，这使它们成为用作发酵剂的有力候选菌株。
