Sun Xiaoyan, Zhou Lei, Zhao Wenying
Institute of Process System Engineering, Qingdao University of Science & Technology, Qingdao, Shandong 266042, PR China.
Institute of Process System Engineering, Qingdao University of Science & Technology, Qingdao, Shandong 266042, PR China.
Bioelectrochemistry. 2022 Jun;145:108104. doi: 10.1016/j.bioelechem.2022.108104. Epub 2022 Mar 19.
Herein, a sensitive immunosensor for the rapid detection of Dibutyl phthalate (DBP) small molecules was developed. Its principle is based on Au@Pt/Polyethyleneimine (PEI)-rGO and hybridization chain reaction signal amplification system. First, Au@Pt/PEI-rGO modified electrode is used as a signal amplification platform and the antibody is fixed. In the presence of target DBP, it competes with DBP-bovine serum albumin-S for binding antibody. Finally, the DNA concatemer carrying a large number of signal molecules methylene blue combines with its S to produce a reduced electrochemical signal. Under the most reasonable conditions, this immunosensor showed a good linear range from1 pg mL to 0.1 μg mLwith a low detection limit of 0.276 pg mL. It has been verified that this immunosensor has good selectivity, reproducibility and stability. The designed biosensing solution will open up a new path for DBP monitoring and other small molecule targets in the food field.
在此，开发了一种用于快速检测邻苯二甲酸二丁酯（DBP）小分子的灵敏免疫传感器。其原理基于金@铂/聚乙烯亚胺（PEI）-还原氧化石墨烯（rGO）和杂交链式反应信号放大系统。首先，将金@铂/PEI-rGO修饰电极用作信号放大平台并固定抗体。在目标DBP存在的情况下，它与DBP-牛血清白蛋白-S竞争结合抗体。最后，携带大量信号分子亚甲蓝的DNA串联体与其S结合产生降低的电化学信号。在最合理的条件下，该免疫传感器显示出从1 pg/mL到0.1 μg/mL的良好线性范围，检测限低至0.276 pg/mL。已证实该免疫传感器具有良好的选择性、重现性和稳定性。所设计的生物传感解决方案将为食品领域中DBP监测及其他小分子目标开辟一条新途径。
