Li Mengjiao, Yu Qingjie, Zheng Meie, Jiang Rongrong, Zhu Hongda, Guo Huiling, Sun Hongmei, Liu Mingxing
Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), School of Biological Engineering and Food, Hubei University of, Technology, Wuhan, 430068, PR China.
Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, 430068, PR China.
Nanoscale. 2023 Feb 23;15(8):4063-4070. doi: 10.1039/d2nr05787e.
A label-free electrochemical immunosensor for high-sensitive detection of β-amyloid 1-42 (Aβ 1-42) was constructed based on Au-modified B, S, and N co-doped reduced graphene oxide (Au-BSN-rGO). The electronic structure of Au-BSN-rGO was investigated by first-principles calculations, which showed that the band gap of graphene was opened, thus improving its electrical conductivity. Moreover, Au-BSN-rGO was successfully prepared and characterized, and the obtained results discovered that it could be used as a signal amplifier for immunosensors due to the advantages of the good electrochemical characteristics and enormous surface area of BSN-rGO and the accelerated electron transfer ability of Au NPs. Furthermore, the label-free electrochemical immunosensor had a linear detection range of 0.1 pg mL-10 ng mL and a detection limit of 0.072 pg mL, and it had good specificity, stability, and reproducibility. Also, this immunosensor showed recoveries of 89%-109% with an RSD of 2.61%-4.19% for detecting Aβ 1-42 in actual sample analysis. Therefore, the label-free electrochemical immunosensor based on Au-BSN-rGO should have a promising clinical application prospect for detecting Aβ 1-42.
基于金修饰的硼、硫、氮共掺杂还原氧化石墨烯（Au-BSN-rGO）构建了一种用于高灵敏度检测β-淀粉样蛋白1-42（Aβ 1-42）的无标记电化学免疫传感器。通过第一性原理计算研究了Au-BSN-rGO的电子结构，结果表明石墨烯的带隙被打开，从而提高了其导电性。此外，成功制备并表征了Au-BSN-rGO，所得结果表明，由于BSN-rGO良好的电化学特性、巨大的表面积以及金纳米颗粒加速电子转移的能力，它可作为免疫传感器的信号放大器。此外，该无标记电化学免疫传感器的线性检测范围为0.1 pg mL-10 ng mL，检测限为0.072 pg mL，具有良好的特异性、稳定性和重现性。在实际样品分析中，该免疫传感器检测Aβ 1-42的回收率为89%-109%，相对标准偏差为2.61%-4.19%。因此，基于Au-BSN-rGO的无标记电化学免疫传感器在检测Aβ 1-42方面应具有广阔的临床应用前景。
