School of Food Science and Technology, Henan University of Technology, Zhengzhou, Henan 450001, PR China.
School of Food Science and Technology, Henan University of Technology, Zhengzhou, Henan 450001, PR China.
Bioelectrochemistry. 2022 Feb;143:107955. doi: 10.1016/j.bioelechem.2021.107955. Epub 2021 Sep 24.
In this work, we design an immunosensor for zearalenone (ZEN) detection with PEI-rGO/Pt@Au NRs nanocomposite as the modification material. PEI-rGO/Pt@Au NRs nanocomposite have good stability, conductivity and a large specific surface area, so they are chosen as the substrate material for the modified electrode, which is beneficial in improving the detection performance of the sensor. When antibody binds to ZEN, the current signal decreases, and the response signal changes after ZEN incubation, recorded by differential pulse voltammetry (DPV) methods. Under the optimised conditions, the electrochemical response of the constructed immunosensor shows a linear relation to a wide concentration range from 1 pg/mL to 1 × 10 pg/mL with a detection limit of 0.02 pg/mL. Additionally, the proposed electrochemical immunosensor has high selectivity, good stability and great potential for the trace detection of ZEN in real samples.
在这项工作中，我们设计了一种基于聚醚酰亚胺-还原氧化石墨烯/铂@金纳米棒（PEI-rGO/Pt@Au NRs）复合材料的免疫传感器，用于玉米赤霉烯酮（ZEN）的检测。PEI-rGO/Pt@Au NRs 纳米复合材料具有良好的稳定性、导电性和较大的比表面积，因此被选择作为修饰电极的基底材料，这有利于提高传感器的检测性能。当抗体与 ZEN 结合时，电流信号减小，孵育 ZEN 后，通过差分脉冲伏安法（DPV）记录响应信号的变化。在优化条件下，所构建的免疫传感器的电化学响应呈现出从 1 pg/mL 到 1×10 pg/mL 宽浓度范围内的线性关系，检测限为 0.02 pg/mL。此外，该电化学免疫传感器具有高选择性、良好的稳定性，在实际样品中痕量 ZEN 的检测方面具有很大的潜力。
