Wang Wei
MOE Key Laboratory of Material Physics and Chemistry under Extraordinary Conditions, School of Physical Science and Technology, Northwestern Polytechnical University, Xi'an 710129, China.
State Key Laboratory of ASIC and Systems, Fudan University, Shanghai 200433, China.
Micromachines (Basel). 2022 Mar 20;13(3):484. doi: 10.3390/mi13030484.
Electro-wetting-on-dielectric (EWOD) enables the manipulation of droplets on a two-dimensional surface, which provides a versatile technique for digital microfluidics at a micro- or nano-scale. However, the deficiency of the dispensing precision has long limited its applications in micro total analysis systems (μ-TAS) where the accuracy of assays is largely determined by the volume control of the reagent dosing. This paper proposes optimum electrode designs and carries out characterization experiments to demonstrate the reproducibility of on-chip droplet generation with no extra external apparatus. The coefficient variation of the volumes of consecutively dispensed droplets from a non-refilling reservoir can be limited to below 0.3%, indicating the validity of the new electrode structure in practical applications.
介电电泳（EWOD）能够在二维表面上操纵液滴，这为微纳尺度的数字微流控提供了一种通用技术。然而，长期以来，分配精度的不足限制了它在微全分析系统（μ-TAS）中的应用，在该系统中，分析的准确性很大程度上取决于试剂加样的体积控制。本文提出了优化的电极设计，并进行了表征实验，以证明无需额外外部设备即可在芯片上产生液滴的可重复性。从非再填充储液器连续分配的液滴体积的变异系数可限制在0.3%以下，表明新电极结构在实际应用中的有效性。
