• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种能引发增强的T细胞介导免疫反应的重组RB51疫苗的设计与特性分析

Design and Characterization of a Recombinant RB51 Vaccine That Elicits Enhanced T Cell-Mediated Immune Response.

作者信息

Sarmadi Mahdieh, Gheibi Azam, Khanahmad Hossein, Khorramizadeh Mohammad Reza, Hejazi Seyed Hossein, Zahedi Noushin, Mianesaz Hamidreza, Kashfi Khosrow

机构信息

Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.

Department of Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences (TUMS), Tehran 14177-55469, Iran.

出版信息

Vaccines (Basel). 2022 Mar 3;10(3):388. doi: 10.3390/vaccines10030388.

DOI:10.3390/vaccines10030388
PMID:35335018
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8950781/
Abstract

vaccines help control bovine brucellosis. The RB51 strain is a live attenuated vaccine with low side effects compared with other live attenuated brucellosis vaccines, but it provides insufficient protective efficacy. Cell-mediated immune responses are critical in resistance against intracellular bacterial infections. Therefore, we hypothesized that the listeriolysin O (LLO) expression of , BAX, and SMAC apoptotic proteins in strain RB51 could enhance vaccine efficacy and safety. RB51 was transformed separately with two broad-host-range plasmids (pbbr1ori-LLO and pBlu-mLLO-BAX-SMAC) constructed from our recent work. pbbr1ori-LLO contains LLO, and pBlu-mLLO-BAX-SMAC contains the mutant LLO and BAX-SMAC fusion gene. The murine macrophage-like cell line J774A.1 was infected with the RB51 recombinant strain containing pBlu-mLLO-BAX-SMAC, RB51 recombinant strain containing LLO, and RB51 strain. The bacterial cytotoxicity and survival and apoptosis of host cells contaminated with our two strain types-RB51 recombinants or the parental RB51-were assessed. Strain RB51 expressing mLLO and BAX-SMAC was tested in BALB/c mice and a cell line for enhanced modulation of IFN-γ production. LDH analysis showed that the RB51-mLLO-BAX-SMAC and RB51-LLO strains expressed higher cytotoxicity in J774A.1 cells than RB51. In addition, RB51 recombinants had lower macrophage survival rates and caused higher levels of apoptosis and necrosis. Mice vaccinated with the RB51 recombinant containing mLLO-BAX-SMAC showed an enhanced Th1 immune response. This enhanced immune response is primarily due to bacterial endosome escape and bacterial antigens, leading to improved apoptosis and cross-priming. This potentially enhanced TCD- and T cell-mediated immunity leads to the increased safety and potency of the RB51 recombinant (RB51 mLLO-BAX-SMAC) as a vaccine candidate against .

摘要

疫苗有助于控制牛布鲁氏菌病。RB51菌株是一种减毒活疫苗，与其他减毒布鲁氏菌病疫苗相比副作用较小，但提供的保护效力不足。细胞介导的免疫反应在抵抗细胞内细菌感染中至关重要。因此，我们假设RB51菌株中李斯特菌溶血素O（LLO）、BAX和SMAC凋亡蛋白的表达可以提高疫苗的效力和安全性。分别用我们近期工作构建的两种广宿主范围质粒（pbbr1ori-LLO和pBlu-mLLO-BAX-SMAC）转化RB51。pbbr1ori-LLO含有LLO，pBlu-mLLO-BAX-SMAC含有突变型LLO和BAX-SMAC融合基因。用含有pBlu-mLLO-BAX-SMAC的RB51重组菌株、含有LLO的RB51重组菌株和RB51菌株感染小鼠巨噬细胞样细胞系J774A.1。评估了被我们的两种菌株类型（RB51重组体或亲本RB51）污染的宿主细胞的细菌细胞毒性以及存活和凋亡情况。在BALB/c小鼠和细胞系中测试了表达mLLO和BAX-SMAC的RB51菌株对IFN-γ产生的增强调节作用。乳酸脱氢酶分析表明，RB51-mLLO-BAX-SMAC和RB51-LLO菌株在J774A.1细胞中的细胞毒性高于RB51。此外，RB51重组体的巨噬细胞存活率较低，导致更高水平的凋亡和坏死。用含有mLLO-BAX-SMAC的RB51重组体接种的小鼠表现出增强的Th1免疫反应。这种增强的免疫反应主要是由于细菌内体逃逸和细菌抗原，导致凋亡改善和交叉启动。这种潜在增强的TCD和T细胞介导的免疫导致RB51重组体（RB51 mLLO-BAX-SMAC）作为抗……疫苗候选物的安全性和效力增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/222a/8950781/0a90765b9980/vaccines-10-00388-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/222a/8950781/d127aeb1ba31/vaccines-10-00388-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/222a/8950781/f0a8dda72b37/vaccines-10-00388-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/222a/8950781/253cfff74a77/vaccines-10-00388-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/222a/8950781/0a90765b9980/vaccines-10-00388-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/222a/8950781/d127aeb1ba31/vaccines-10-00388-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/222a/8950781/f0a8dda72b37/vaccines-10-00388-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/222a/8950781/253cfff74a77/vaccines-10-00388-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/222a/8950781/0a90765b9980/vaccines-10-00388-g005.jpg

相似文献

1
Design and Characterization of a Recombinant RB51 Vaccine That Elicits Enhanced T Cell-Mediated Immune Response.一种能引发增强的T细胞介导免疫反应的重组RB51疫苗的设计与特性分析
Vaccines (Basel). 2022 Mar 3;10(3):388. doi: 10.3390/vaccines10030388.
2
Characterization of recombinant B. abortus strain RB51SOD toward understanding the uncorrelated innate and adaptive immune responses induced by RB51SOD compared to its parent vaccine strain RB51.重组布氏杆菌 RB51SOD 特性分析，旨在了解与亲本疫苗株 RB51 相比，RB51SOD 诱导的先天和适应性免疫反应的非相关性。
Front Cell Infect Microbiol. 2011 Nov 25;1:10. doi: 10.3389/fcimb.2011.00010. eCollection 2011.
3
Brucella abortus strain RB51 as a vector for heterologous protein expression and induction of specific Th1 type immune responses.布鲁氏菌流产菌株RB51作为异源蛋白表达载体及特异性Th1型免疫应答诱导剂。
Infect Immun. 2000 Jun;68(6):3290-6. doi: 10.1128/IAI.68.6.3290-3296.2000.
4
Booster vaccination with safe, modified, live-attenuated mutants of Brucella abortus strain RB51 vaccine confers protective immunity against virulent strains of B. abortus and Brucella canis in BALB/c mice.用安全的、经过改造的流产布鲁氏菌RB51疫苗减毒活突变体进行加强免疫，可使BALB/c小鼠对流产布鲁氏菌和犬布鲁氏菌的强毒株产生保护性免疫。
Microbiology (Reading). 2015 Nov;161(11):2137-48. doi: 10.1099/mic.0.000170. Epub 2015 Sep 3.
5
Enhanced efficacy of recombinant Brucella abortus RB51 vaccines against B. melitensis infection in mice.重组流产布鲁氏菌RB51疫苗对小鼠感染羊布鲁氏菌的增强疗效。
Vet Microbiol. 2004 Sep 8;102(3-4):237-45. doi: 10.1016/j.vetmic.2004.07.001.
6
Overexpression of protective antigen as a novel approach to enhance vaccine efficacy of Brucella abortus strain RB51.保护性抗原的过表达作为一种增强流产布鲁氏菌RB51疫苗效力的新方法。
Infect Immun. 2000 Jun;68(6):3286-9. doi: 10.1128/IAI.68.6.3286-3289.2000.
7
Induction of antigen-specific Th1-type immune responses by gamma-irradiated recombinant Brucella abortus RB51.经γ射线照射的重组布鲁氏菌RB51诱导抗原特异性Th1型免疫反应
Clin Diagn Lab Immunol. 2005 Dec;12(12):1429-36. doi: 10.1128/CDLI.12.12.1429-1436.2005.
8
Reduced cerebral infection of Neospora caninum in BALB/c mice vaccinated with recombinant Brucella abortus RB51 strains expressing N. caninum SRS2 and GRA7 proteins.用表达犬新孢子虫SRS2和GRA7蛋白的重组流产布鲁氏菌RB51菌株接种的BALB/c小鼠，其大脑中犬新孢子虫感染减少。
Vet Parasitol. 2007 Sep 30;148(3-4):219-30. doi: 10.1016/j.vetpar.2007.06.029. Epub 2007 Jul 24.
9
Caspase-2 mediated apoptotic and necrotic murine macrophage cell death induced by rough Brucella abortus.粗糙布鲁氏菌诱导 Caspase-2 介导热休克和坏死型鼠巨噬细胞死亡
PLoS One. 2009 Aug 28;4(8):e6830. doi: 10.1371/journal.pone.0006830.
10
Overexpression of wbkF gene in Brucella abortus RB51WboA leads to increased O-polysaccharide expression and enhanced vaccine efficacy against B. abortus 2308, B. melitensis 16M, and B. suis 1330 in a murine brucellosis model.wbkF 基因在布鲁氏菌 RB51WboA 中的过表达导致 O-多糖表达增加，并增强了在小鼠布鲁氏菌病模型中对 B. abortus 2308、B. melitensis 16M 和 B. suis 1330 的疫苗效力。
PLoS One. 2019 Mar 11;14(3):e0213587. doi: 10.1371/journal.pone.0213587. eCollection 2019.

引用本文的文献

1
A BtpB-Deleted Brucella Vaccine Strain Provides Dual Advantages: Enhanced Protection and Diagnostic Differentiation Capability in Mice.一种缺失BtpB的布鲁氏菌疫苗株具有双重优势：增强小鼠的保护作用和诊断鉴别能力。
Microb Biotechnol. 2025 Sep;18(9):e70223. doi: 10.1111/1751-7915.70223.

本文引用的文献

1
Characterization of Brucella abortus S19 as a challenge strain for use in a mouse model of brucellosis.布鲁氏菌 S19 特征分析作为布鲁氏菌病小鼠模型的挑战菌株。
Microbes Infect. 2021 May-Jun;23(4-5):104809. doi: 10.1016/j.micinf.2021.104809. Epub 2021 Mar 19.
2
Optimization and Comparison of Different Methods and Factors for Efficient Transformation of RB51strain.RB51菌株高效转化的不同方法和因素的优化与比较
Adv Biomed Res. 2019 May 30;8:37. doi: 10.4103/abr.abr_14_19. eCollection 2019.
3
Overexpression of wbkF gene in Brucella abortus RB51WboA leads to increased O-polysaccharide expression and enhanced vaccine efficacy against B. abortus 2308, B. melitensis 16M, and B. suis 1330 in a murine brucellosis model.
wbkF 基因在布鲁氏菌 RB51WboA 中的过表达导致 O-多糖表达增加，并增强了在小鼠布鲁氏菌病模型中对 B. abortus 2308、B. melitensis 16M 和 B. suis 1330 的疫苗效力。
PLoS One. 2019 Mar 11;14(3):e0213587. doi: 10.1371/journal.pone.0213587. eCollection 2019.
4
Development of new generation of vaccines for .用于……的新一代疫苗的研发
Heliyon. 2018 Dec 26;4(12):e01079. doi: 10.1016/j.heliyon.2018.e01079. eCollection 2018 Dec.
5
Prevalence of in raw milk: An example from Turkey.生牛奶中（此处原文缺失具体内容）的流行情况：以土耳其为例。
Niger J Clin Pract. 2018 Jul;21(7):907-911. doi: 10.4103/njcp.njcp_211_17.
6
Bax, Bak and beyond - mitochondrial performance in apoptosis. Bax、Bak 及其他——细胞凋亡中线粒体的作用
FEBS J. 2018 Feb;285(3):416-431. doi: 10.1111/febs.14186. Epub 2017 Sep 4.
7
Control of animal brucellosis: The Malaysian experience.动物布鲁氏菌病的防控：马来西亚的经验
Asian Pac J Trop Med. 2016 Dec;9(12):1136-1140. doi: 10.1016/j.apjtm.2016.11.007. Epub 2016 Nov 9.
8
Comparison between Immunization Routes of Live Attenuated Salmonella Typhimurium Strains Expressing BCSP31, Omp3b, and SOD of Brucella abortus in Murine Model.在小鼠模型中表达流产布鲁氏菌BCSP31、Omp3b和超氧化物歧化酶的减毒活鼠伤寒沙门氏菌菌株免疫途径的比较
Front Microbiol. 2016 Apr 20;7:550. doi: 10.3389/fmicb.2016.00550. eCollection 2016.
9
Recent advances in Brucella abortus vaccines.布鲁氏菌流产疫苗的最新进展。
Vet Res. 2015 Jul 8;46(1):76. doi: 10.1186/s13567-015-0199-7.
10
Pathogenesis and immunobiology of brucellosis: review of Brucella-host interactions.布鲁氏菌病的发病机制与免疫生物学：布鲁氏菌与宿主相互作用的综述
Am J Pathol. 2015 Jun;185(6):1505-17. doi: 10.1016/j.ajpath.2015.03.003. Epub 2015 Apr 17.