Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, 2015 SW 16th Ave, Gainesville, FL, 32608, USA.
Microbes Infect. 2021 May-Jun;23(4-5):104809. doi: 10.1016/j.micinf.2021.104809. Epub 2021 Mar 19.
The objective of this project was to conduct a feasibility study to determine whether the Brucella abortus S19 vaccine infects and persists in mice and determine whether S19 can be used as a challenge strain for vaccine trial studies. Groups of BALB/c mice were inoculated (intraperitoneally, subcutaneously, intranasally) and euthanized to determine colonization titers in the spleens and lungs. This study showed that S19 does infect and persist in the tissues of mice for 8 weeks and demonstrates that S19 can be used, safely and economically under BSL2 containment, as the challenge strain for future trials to evaluate vaccine efficacy.
本项目的目的是进行一项可行性研究，以确定布鲁氏菌 abortus S19 疫苗是否感染并持续存在于小鼠体内，并确定 S19 是否可用作疫苗试验研究的挑战株。将 BALB/c 小鼠分组（腹腔内、皮下、鼻内）接种并安乐死，以确定脾脏和肺部的定植滴度。本研究表明，S19 确实可以感染并持续存在于小鼠组织中 8 周，并且表明 S19 可以在 BSL2 控制下安全、经济地使用，作为未来评估疫苗效力的试验的挑战株。
