Insecticidal and Enzyme Inhibition Activities of Leaf/Bark Extracts, Fractions, Seed Oil and Isolated Compounds from (L.) Small against Koch.

Aphid, Koch (Hemiptera: Aphididae), is a major sap-sucking insect pest of leguminous crops and also transmits plant viruses, leading to economic yield loss. Indiscriminate and repeated use of insecticides for control of aphid leads to the development of resistance, and is harmful to the environment, non-target organisms, etc. Plant-based extracts/seed oils (SO) are the best alternatives to insecticides. Insecticidal activities of have not been reported against and other insect pests to date. In the current study, the main objective was to study the insecticidal activities of leaf/bark extracts/fractions, seed oil, isolated compounds, and their combinations against . Results showed that, among the extracts, ethanolic bark extract 80% (LC = 5115.98 mg/L) was more effective against . Among fractions, the -hexane fraction of leaves (LC = 425.73 mg/L) and the ethyl acetate fraction of bark (LC = 813.45 mg/L) were promising. Among compounds, gallic acid was the most effective (LC = 1303.68 mg/L) compared to shikimic acid and quercetin. SO (LC = 850.94 mg/L) was superior compared to extracts/fractions/compounds. All the combinations showed toxicity and synergistic activity. Leaf/bark extracts and SO significantly inhibited the AChE and GST activity in . Based on field bio-efficacy, the leaf extract/SO or their combinations can be recommended for the control of aphids.