Entomology Laboratory, Agrotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Molecules. 2022 Mar 18;27(6):1967. doi: 10.3390/molecules27061967.
Aphid, Koch (Hemiptera: Aphididae), is a major sap-sucking insect pest of leguminous crops and also transmits plant viruses, leading to economic yield loss. Indiscriminate and repeated use of insecticides for control of aphid leads to the development of resistance, and is harmful to the environment, non-target organisms, etc. Plant-based extracts/seed oils (SO) are the best alternatives to insecticides. Insecticidal activities of have not been reported against and other insect pests to date. In the current study, the main objective was to study the insecticidal activities of leaf/bark extracts/fractions, seed oil, isolated compounds, and their combinations against . Results showed that, among the extracts, ethanolic bark extract 80% (LC = 5115.98 mg/L) was more effective against . Among fractions, the -hexane fraction of leaves (LC = 425.73 mg/L) and the ethyl acetate fraction of bark (LC = 813.45 mg/L) were promising. Among compounds, gallic acid was the most effective (LC = 1303.68 mg/L) compared to shikimic acid and quercetin. SO (LC = 850.94 mg/L) was superior compared to extracts/fractions/compounds. All the combinations showed toxicity and synergistic activity. Leaf/bark extracts and SO significantly inhibited the AChE and GST activity in . Based on field bio-efficacy, the leaf extract/SO or their combinations can be recommended for the control of aphids.
蚜虫，科赫（半翅目：蚜科），是豆类作物的主要刺吸式害虫，也是植物病毒的传播媒介，导致经济产量损失。为防治蚜虫而不加区分和重复使用杀虫剂会导致抗药性的产生，对环境、非靶标生物等有害。植物提取物/种子油（SO）是杀虫剂的最佳替代品。迄今为止，尚未有报道称对 和其他害虫具有杀虫活性。在当前的研究中，主要目的是研究叶/皮提取物/馏分、种子油、分离化合物及其组合对 的杀虫活性。结果表明，在所测试的提取物中，80%乙醇树皮提取物（LC = 5115.98 mg/L）对 最有效。在馏分中，叶的正己烷馏分（LC = 425.73 mg/L）和树皮的乙酸乙酯馏分（LC = 813.45 mg/L）具有很大的应用潜力。在所测试的化合物中，没食子酸（LC = 1303.68 mg/L）比莽草酸和槲皮素更有效。与提取物/馏分/化合物相比，SO（LC = 850.94 mg/L）的效果更好。所有组合均表现出毒性和协同活性。叶/皮提取物和 SO 显著抑制了 中的 AChE 和 GST 活性。基于田间药效，叶提取物/种子油或其组合可推荐用于防治蚜虫。
