Filler W A, Kemp R M, Ng J C, Hawkes T R, Dixon R A, Smith B E
Eur J Biochem. 1986 Oct 15;160(2):371-7. doi: 10.1111/j.1432-1033.1986.tb09981.x.
The MoFe protein of nitrogenase from Klebsiella pneumoniae contains an iron-molybdenum cofactor, FeMoco, the synthesis or processing of which involves the products of at least five genes, nifQ, nifB, nifN, nifE and nifV. We have detected FeMoco activity in extracts of strains which synthesise neither of the MoFe protein subunits, indicating that FeMoco can be synthesised prior to combination with the MoFe protein polypeptides. Expression of the nifH gene (or a large part of it), was essential for FeMoco activity to be observed either in the presence or in the absence of the MoFe protein subunits. The nifH gene product was not involved in the control of the transcription of other nif gene products known to be involved in FeMoco synthesis or processing, nor was it essential for the stability of performed FeMoco before its combination with the MoFe protein polypeptides.
肺炎克雷伯菌固氮酶的钼铁蛋白含有一个铁钼辅因子FeMoco，其合成或加工涉及至少五个基因nifQ、nifB、nifN、nifE和nifV的产物。我们在既不合成钼铁蛋白亚基的菌株提取物中检测到了FeMoco活性，这表明FeMoco可以在与钼铁蛋白多肽结合之前合成。无论是否存在钼铁蛋白亚基，nifH基因（或其大部分）的表达对于观察到FeMoco活性都是必不可少的。nifH基因产物不参与已知参与FeMoco合成或加工的其他nif基因产物转录的控制，在已形成的FeMoco与钼铁蛋白多肽结合之前，它对于其稳定性也不是必需的。
