Miloloža Martina, Bule Kristina, Prevarić Viktorija, Cvetnić Matija, Ukić Šime, Bolanča Tomislav, Kučić Grgić Dajana
Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia.
Department for Packaging, Recycling and Environmental Protection, University North, 48000 Koprivnica, Croatia.
Polymers (Basel). 2022 Mar 19;14(6):1246. doi: 10.3390/polym14061246.
The harmful effects of microplastics are not yet fully revealed. This study tested harmful effects of polyethylene (PE), polypropylene (PP), polystyrene (PS), polyvinyl chloride (PVC), and polyethylene terephthalate (PET) microplastics were tested. Growth inhibition tests were conducted using three microorganisms with different characteristics: sp., , and . The growth inhibition test with sp. is relatively widely used, while the tests with and were, to our knowledge, applied to microplastics for the first time. The influence of concentration and size of microplastic particles, in the range of 50-1000 mg/L and 200-600 µm, was tested. Determined inhibitions on all three microorganisms confirmed the hazardous potential of the microplastics used. Modeling of the inhibition surface showed the increase in harmfulness with increasing concentration of the microplastics. Particle size showed no effect for with PE, PP and PET, with PS, and with PP. In the remaining cases, higher inhibitions followed a decrease in particle size. The exception was sp. with PS, where the lowest inhibitions were obtained at 400 µm. Finally, among the applied tests, the test with proved to be the most sensitive to microplastics.
微塑料的有害影响尚未完全显现。本研究测试了聚乙烯（PE）、聚丙烯（PP）、聚苯乙烯（PS）、聚氯乙烯（PVC）和聚对苯二甲酸乙二酯（PET）微塑料的有害影响。使用三种具有不同特性的微生物进行生长抑制试验： 菌、 菌和 菌。 菌的生长抑制试验应用相对广泛，而据我们所知， 菌和 菌的试验首次应用于微塑料。测试了微塑料颗粒浓度和尺寸在50 - 1000 mg/L和200 - 600 µm范围内的影响。对所有三种微生物的抑制作用测定证实了所用微塑料的潜在危害。抑制表面建模显示，随着微塑料浓度的增加，危害性增加。对于PE、PP和PET的 菌、PS的 菌以及PP的 菌，粒径没有影响。在其余情况下，粒径减小会导致更高的抑制作用。例外的是PS的 菌，在400 µm时抑制作用最低。最后，在所应用的试验中， 菌的试验被证明对微塑料最敏感。
