Iuchi Hiroyuki, Ohori Junichiro, Matsuzaki Hisahiro, Tokushige Takeshi, Toge Sakiko, Yamashita Masaru
Department of Otolaryngology, Head and Neck Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan.
Microorganisms. 2022 Feb 28;10(3):527. doi: 10.3390/microorganisms10030527.
Phosphorylcholine (PC) is a structural component of various pathogens and is involved in bacterial adhesion via the platelet-activating factor receptor (PAF-R). In this study, we investigated how PC expression affects cell adhesion and invasion of (). Eight clinical strains of were cultured, and PC expression was measured using fluorescence-activated cell sorting. Bacterial adherence and invasion were examined using Detroit 562 cells. An anti-PC-specific monoclonal antibody (TEPC-15) was used to inhibit bacterial PC, and a PAF-R antagonist (ABT-491) was used to inhibit cellular PAF-R. The gene was amplified by the polymerase chain reaction with the standard primers. The level of PC expressed on the surfaces differed in each strain and differed even in the same genotype. Adherence assay experiments showed a significant negative correlation between TEPC-15 and ABT-491 inhibitory effects and PC expression in . Similarly, intracellular invasion assay experiments showed a significant negative correlation between TEPC-15 and ABT-491 inhibitory effects and PC expression in . This study suggests that is involved in cell adhesion and invasion by PC.
磷酰胆碱（PC）是多种病原体的结构成分，可通过血小板活化因子受体（PAF-R）参与细菌黏附。在本研究中，我们调查了PC表达如何影响（）的细胞黏附和侵袭。培养了8株临床（）菌株，使用荧光激活细胞分选术测量PC表达。使用底特律562细胞检测细菌黏附和侵袭情况。使用抗PC特异性单克隆抗体（TEPC-15）抑制细菌PC，使用PAF-R拮抗剂（ABT-491）抑制细胞PAF-R。用标准引物通过聚合酶链反应扩增（）基因。各菌株表面表达的PC水平不同，即使在相同的（）基因型中也有所不同。黏附试验实验表明，TEPC-15和ABT-491的抑制作用与（）中PC表达之间存在显著负相关。同样，细胞内侵袭试验实验表明，TEPC-15和ABT-491的抑制作用与（）中PC表达之间存在显著负相关。本研究表明，（）通过PC参与细胞黏附和侵袭。 （注：原文中部分括号内容缺失，翻译时保留原样）
