Ma Hao, Wang Weiwei, Wang Zhenyu, Tan Zhongfang, Qin Guangyong, Wang Yanping, Pang Huili
School of Physics and Microelectronics, Zhengzhou University, Zhengzhou 450052, China.
Henan Key Lab Ion Beam Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, China.
Microorganisms. 2022 Feb 28;10(3):530. doi: 10.3390/microorganisms10030530.
This study assessed the effects of () in combination with protease on fermentation characteristics and microbial communities during ensiling and aerobic exposure phases of soybean meal (SBM). In this study, response surface methodology (RSM) was used to optimize the optimal growth conditions of ZZUPF95, which produced protease, and fermented SBM under the optimal fermentation conditions. The fermentation test was divided into four groups as follows: CK (Control check), ZZUPF95, Protease and ZZUPF95+Protease groups. Results showed that the best medium ratio of ZZUPF95 was glucose 1%, peptone 2%, inorganic salt 1.47%; fermentation time 36 h, inoculation amount 10%, ratio of material to water 1:1 is the optimal fermentation scheme; after fermentation and aerobic exposure treatment, ZZUPF95 and ZZUPF95 + Protease group can reduce the pH of feed, improve the content of lactic acid in the fermentation system, and have the effect of inhibiting the reproduction of pathogenic bacteria, increasing the content of crude protein and ether extract, and degrading crude fiber; the microbial community of SBM were changed after fermentation and aerobic exposure. This study explored the changes of fermentation quality of SBM, which has certain theoretical value to improve the fermentation mode and storage of SBM.
本研究评估了()与蛋白酶联合使用对豆粕(SBM)青贮和有氧暴露阶段发酵特性及微生物群落的影响。本研究采用响应面法(RSM)优化产蛋白酶的ZZUPF95的最佳生长条件,并在最佳发酵条件下发酵SBM。发酵试验分为四组:CK(对照)、ZZUPF95、蛋白酶和ZZUPF95 + 蛋白酶组。结果表明,ZZUPF95的最佳培养基配比为葡萄糖1%、蛋白胨2%、无机盐1.47%;发酵时间36 h、接种量10%、料水比1:1为最佳发酵方案;经过发酵和有氧暴露处理后,ZZUPF95和ZZUPF95 + 蛋白酶组可降低饲料pH值,提高发酵体系中乳酸含量,具有抑制病原菌繁殖、提高粗蛋白和乙醚提取物含量以及降解粗纤维的作用;发酵和有氧暴露后SBM的微生物群落发生了变化。本研究探讨了SBM发酵品质的变化,对改进SBM的发酵方式和储存具有一定的理论价值。