Puhm Marge, Ainelo Hanna, Kivisaar Maia, Teras Riho
Institute of Molecular and Cell Biology, University of Tartu, 51010 Tartu, Estonia.
Microorganisms. 2022 Mar 14;10(3):618. doi: 10.3390/microorganisms10030618.
Extracellular factors and growth conditions can affect the formation and development of bacterial biofilms. The biofilm of has been studied for decades, but so far, little attention has been paid to the components of the medium that may affect the biofilm development in a closed system. It is known that Fis strongly enhances biofilm in complete LB medium. However, this is not the case in the defined M9 medium, which led us to question why the bacterium behaves differently in these two media. Detailed analysis of the individual medium components revealed that tryptone as the LB proteinaceous component maintains biofilm in its older stages. Although the growth parameters of planktonic cells were similar in the media containing tryptone or an equivalent concentration of amino acids, only the tryptone had a positive effect on the mature biofilm of the wild type strain of . Thus, the peptides in the environment may influence mature biofilm as a structural factor and not only as an energy source. Testing the effect of other biopolymers on biofilm formation showed variable results even for polymers with a similar charge, indicating that biopolymers can affect biofilm through a number of bacterial factors.
细胞外因子和生长条件会影响细菌生物膜的形成与发育。对生物膜的研究已有数十年，但迄今为止，在封闭系统中，很少有人关注可能影响生物膜发育的培养基成分。已知Fis在完全LB培养基中能强烈增强生物膜形成。然而，在限定的M9培养基中情况并非如此，这使我们质疑为何该细菌在这两种培养基中的表现不同。对各培养基成分的详细分析表明，作为LB含蛋白质成分的胰蛋白胨在生物膜发育后期维持其结构。尽管在含有胰蛋白胨或同等浓度氨基酸的培养基中浮游细胞的生长参数相似，但只有胰蛋白胨对野生型菌株的成熟生物膜有积极影响。因此，环境中的肽可能作为一种结构因子而非仅作为能量来源影响成熟生物膜。测试其他生物聚合物对生物膜形成的影响，即使对于电荷相似的聚合物也显示出不同结果，表明生物聚合物可通过多种细菌因子影响生物膜形成。
