Bagno Flávia F, Andrade Luis A F, Sérgio Sarah A R, Parise Pierina L, Toledo-Teixeira Daniel A, Gazzinelli Ricardo T, Fernandes Ana P S M, Teixeira Santuza M R, Granja Fabiana, Proença-Módena José L, da Fonseca Flavio G
Centro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, Brazil.
Laboratory of Emerging Viruses, Department of Genetics, Evolution, Microbiology and Immunology, University of Campinas, Campinas 13083-862, Brazil.
Viruses. 2022 Mar 2;14(3):510. doi: 10.3390/v14030510.
Previous studies have indicated that antibody responses can be robustly induced after the vaccination in individuals previously infected by SARS-CoV-2. To evaluate anti-SARS-CoV-2 humoral responses in vaccinated individuals with or without a previous history of COVID-19, we compared levels of anti-SARS-CoV-2 antibodies in the sera from 21 vaccinees, including COVID-19-recovered or -naïve individuals in different times, before and after immunization with an inactivated COVID-19 vaccine. Anti-SARS-CoV-2-specific antibodies elicited after COVID-19 and/or immunization with an inactivated vaccine were measured by ELISA and Plaque Reduction Neutralizing assays. Antibody kinetics were consistently different between the two vaccine doses for naïve individuals, contrasting with the SARS-CoV-2-recovered subjects in which we observed no additional increase in antibody levels following the second dose. Sera from SARS-CoV2-naïve individuals had no detectable neutralizing activity against lineage B.1 SARS-CoV-2 or Gamma variant five months after the second vaccine dose. Contrarily, SARS-CoV-2-recovered subjects retained considerable neutralizing activity against both viruses. We conclude that a single inactivated SARS-CoV-2 vaccine dose may be sufficient to induce protective antibody responses in individuals with previous history of SARS-CoV-2 infection.
先前的研究表明，在曾感染过SARS-CoV-2的个体中，接种疫苗后可强烈诱导抗体反应。为了评估有或没有COVID-19既往病史的接种疫苗个体的抗SARS-CoV-2体液反应，我们比较了21名接种者血清中抗SARS-CoV-2抗体的水平，这些接种者包括不同时期的COVID-19康复者或未感染者，在接种灭活COVID-19疫苗之前和之后。通过ELISA和蚀斑减少中和试验测量COVID-19感染后和/或接种灭活疫苗后产生的抗SARS-CoV-2特异性抗体。对于未感染过的个体，两剂疫苗后的抗体动力学始终不同，这与SARS-CoV-2康复者形成对比，在康复者中我们观察到第二剂疫苗后抗体水平没有进一步增加。在第二剂疫苗接种五个月后，未感染过SARS-CoV-2的个体的血清对B.1谱系SARS-CoV-2或Gamma变体没有可检测到的中和活性。相反，SARS-CoV-2康复者对这两种病毒都保留了相当的中和活性。我们得出结论，对于有SARS-CoV-2感染既往史的个体，单剂灭活SARS-CoV-2疫苗可能足以诱导保护性抗体反应。
