Vitalant Research Institute, 270 Masonic Ave., San Francisco, CA 94118, USA.
Department of Laboratory Medicine, University of California, San Francisco, CA 94118, USA.
Viruses. 2022 Mar 19;14(3):638. doi: 10.3390/v14030638.
Idiopathic chronic diarrhea (ICD) is a little understood common clinical problem in captive rhesus macaques claiming 33% of medical culls unrelated to research. The eukaryotic virome in digestive tract tissues collected at necropsy from nine animals with ICD was characterized using viral metagenomics. We compared the distribution of viral reads in tissues and mucosal scrapings from the stomach, duodenum, jejunum, ileum, and the proximal, transverse, and distal colons. In situ hybridization (ISH) using viral probes were performed on fixed tissues. Deep sequencing revealed multiple viruses in the and family. Tissues and mucosal scraping from the same locations showed closely related viral reads contents while different gut tissues from the same animal varied widely. ISH showed punctuated staining for both RNA and DNA viruses in the distal colon. Parvovirus staining was also detected in the stomach/duodenum/jejunum in distinct oval-shaped structures. The location of enteric viral nucleic acid differed widely between different viral families and along the length of the digestive tract.
特发性慢性腹泻（ICD）是一种在圈养恒河猴中常见但尚未被充分了解的临床问题，导致 33%的与研究无关的淘汰与 ICD 有关。通过病毒宏基因组学，对来自 9 只患有 ICD 的动物尸检时收集的消化道组织中的真核病毒组进行了特征描述。我们比较了组织和胃、十二指肠、空肠、回肠以及结肠近端、横结肠和远端黏膜刮片中的病毒读段分布。对固定组织进行了病毒探针原位杂交（ISH）。深度测序显示 和 科有多种病毒。同一部位的组织和黏膜刮片显示出密切相关的病毒读段含量，而同一动物的不同肠道组织则差异很大。ISH 显示在远端结肠有明显的点状 RNA 和 DNA 病毒染色。在胃/十二指肠/空肠中也检测到细小病毒点状染色。肠病毒核酸的位置在不同的病毒科之间以及消化道的长度上差异很大。
