Powell R C, Millar R P, King J A
Gen Comp Endocrinol. 1986 Jul;63(1):77-85. doi: 10.1016/0016-6480(86)90184-x.
Immunoreactive and biologically active gonadotropin-releasing hormones (GnRHs) in dogfish (Poroderma africanum) and teleost (Coris julis) brain extracts were studied by high-performance liquid chromatography (HPLC), radioimmunoassay with region-specific antisera, and assessment of luteinizing hormone (LH)-releasing activity in a chicken dispersed pituitary cell bioassay. In dogfish brain extract, seven GnRH molecular forms with LH-releasing activity were demonstrated. Three of these forms coeluted with synthetic mammalian GnRH; His5,Trp7,Tyr8-GnRH; and Trp7,Leu8-GnRH on HPLC. The peaks coincident with His5,Trp7,Tyr8-GnRH and Trp7,Leu8-GnRH had immunological and biological properties identical to those of the synthetic peptides. However, the molecular form coeluting with mammalian GnRH had immunological and biological properties different from those of mammalian GnRH and is thus a novel molecular variant of GnRH. The four remaining forms are also novel GnRHs or structurally unrelated peptides with LH-releasing activity. Dogfish systemic blood contained immunoreactive GnRH. In teleost brain extract, three biologically active GnRH forms with LH-releasing activity were present. The major peak of GnRH immunoreactivity coeluted with Trp7,Leu8-GnRH, and a second immunoreactive form coeluted with His5,Trp7,Tyr8-GnRH. The third biologically active peak is a novel, early-eluting molecular variant of GnRH or a structurally unrelated peptide with LH-releasing activity.
通过高效液相色谱法（HPLC）、使用区域特异性抗血清的放射免疫测定法以及在鸡分散垂体细胞生物测定中评估促黄体生成素（LH）释放活性，对非洲豹鲨（Poroderma africanum）和隆头鱼（Coris julis）脑提取物中的免疫反应性和生物活性促性腺激素释放激素（GnRHs）进行了研究。在非洲豹鲨脑提取物中，证实了七种具有LH释放活性的GnRH分子形式。其中三种形式与合成的哺乳动物GnRH共洗脱；His5、Trp7、Tyr8 - GnRH；以及Trp7、Leu8 - GnRH在HPLC上。与His5、Trp7、Tyr8 - GnRH和Trp7、Leu8 - GnRH重合的峰具有与合成肽相同的免疫和生物学特性。然而，与哺乳动物GnRH共洗脱的分子形式具有与哺乳动物GnRH不同的免疫和生物学特性，因此是GnRH的一种新型分子变体。其余四种形式也是具有LH释放活性的新型GnRH或结构无关的肽。非洲豹鲨的全身血液中含有免疫反应性GnRH。在隆头鱼脑提取物中，存在三种具有LH释放活性的生物活性GnRH形式。GnRH免疫反应性的主要峰与Trp7、Leu8 - GnRH共洗脱，第二种免疫反应形式与His5、Trp7、Tyr8 - GnRH共洗脱。第三个生物活性峰是GnRH的一种新型、早期洗脱的分子变体或具有LH释放活性的结构无关的肽。
