鸵鸟（鸵鸟属骆驼）下丘脑和下丘脑外脑组织中谷氨酰胺8-促性腺激素释放激素及组氨酸5、色氨酸7、酪氨酸8-促性腺激素释放激素的鉴定。

Identification of Gln8-GnRH and His5,Trp7,Tyr8-GnRH in the hypothalamus and extrahypothalamic brain of the ostrich (Struthio camelus).

作者信息

Powell R C, Jach H, Millar R P, King J A

出版信息

Peptides. 1987 Jan-Feb;8(1):185-90. doi: 10.1016/0196-9781(87)90185-9.

Abstract

Gonadotropin-releasing hormone (GnRH) molecular forms were studied in extracts of ostrich hypothalamus and extrahypothalamic brain using high performance liquid chromatography, radioimmunoassay with region-specific antisera and assessment of luteinizing hormone (LH)-releasing activity using chicken dispersed pituitary cells. Two molecular forms of GnRH with chromatographic, immunological and biological properties identical to those of Gln8-GnRH and His5,Trp7,Tyr8-GnRH were demonstrated in both the hypothalamic and extrahypothalamic brain extracts. A greater proportion of His5,Trp7,Tyr8-GnRH was present in the hypothalamus than in extrahypothalamic brain. It is likely that these two forms of GnRH are present in all bird species, since the chicken and the ostrich have evolved separately.

摘要

使用高效液相色谱法、针对特定区域抗血清的放射免疫分析法以及利用鸡分散垂体细胞评估促黄体生成素（LH）释放活性的方法，对鸵鸟下丘脑和下丘脑外脑组织提取物中的促性腺激素释放激素（GnRH）分子形式进行了研究。在下丘脑和下丘脑外脑组织提取物中均证实了两种GnRH分子形式，其色谱、免疫和生物学特性与谷氨酰胺8 - GnRH和组氨酸5、色氨酸7、酪氨酸8 - GnRH相同。与下丘脑外脑组织相比，下丘脑中有更大比例的组氨酸5、色氨酸7、酪氨酸8 - GnRH。由于鸡和鸵鸟是分别进化的，所以这两种GnRH形式可能存在于所有鸟类中。