Department of Cardiovascular Surgery, ZhongNan Hospital of Wuhan University, Wuhan, Hubei 430060, China.
Hubei Key Laboratory of Biomass Resource Chemistry and Environmental Biotechnology, Hubei Engineering Center of Natural Polymers-based Medical Materials, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
Carbohydr Polym. 2022 Jun 15;286:119269. doi: 10.1016/j.carbpol.2022.119269. Epub 2022 Feb 28.
The morbidity of a diverse array of esophageal diseases has been increasing in recent years, and postoperative bacterial infection is a common potential risk. Current research on artificial esophageal stents is lacking and less than satisfactory. Artificial materials with excellent mechanical, antimicrobial, and cytocompatible properties at the same time still have great potential. In this study, chitosan (CS) and collagen (COL) were deposited on nylon 6 (N6)/silk fibroin (SF) composite nanofibers using layer-by-layer self-assembly (LBL). The obtained nanofibrous mats were characterized, and the results indicated that the surface morphology and hydrophilicity of the composite nanofibrous mats were obviously improved by the deposition of CS and COL. The mechanical properties of the composite nanofibrous mats were also improved after LBL modification. In addition, the LBL-structured nanofibrous mats possessed excellent antibacterial activity, reaching more than 90% inhibition of gram-positive and negative bacteria. The results of cell experiments indicated that the nanofibrous mats modified by CS and COL had excellent biocompatibility for promoting human esophageal epithelial cell adhesion and proliferation. In vivo animal experiments showed that the LBL-structured nanofibrous mats were beneficial to the healing of esophageal defects compared with the control group. In summary, LBL-structured nanofibrous mats are very promising materials for esophageal replacement to reduce the incidence of postoperative complications.
近年来，各种食管疾病的发病率不断上升，术后细菌感染是一种常见的潜在风险。目前对人工食管支架的研究还不够完善，不尽如人意。同时具有优异的机械性能、抗菌性能和细胞相容性的人工材料仍具有很大的潜力。在这项研究中，壳聚糖（CS）和胶原蛋白（COL）通过层层自组装（LBL）沉积在尼龙 6（N6）/丝素（SF）复合纳米纤维上。对所得纳米纤维垫进行了表征，结果表明，CS 和 COL 的沉积明显改善了复合纳米纤维垫的表面形貌和亲水性。LBL 修饰后，复合纳米纤维垫的力学性能也得到了提高。此外，LBL 结构的纳米纤维垫具有优异的抗菌活性，对革兰氏阳性菌和革兰氏阴性菌的抑制率超过 90%。细胞实验结果表明，CS 和 COL 修饰的纳米纤维垫对促进人食管上皮细胞黏附和增殖具有优异的生物相容性。体内动物实验表明，与对照组相比，LBL 结构的纳米纤维垫有利于食管缺损的愈合。总之，LBL 结构的纳米纤维垫是一种很有前途的食管替代材料，可以降低术后并发症的发生率。
