Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, China; School of Stomatology, Southern Medical University, Guangzhou, 510515, China.
Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, School of Resource and Environmental Science, Wuhan University, Wuhan, 430079, China; The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
Carbohydr Polym. 2021 Jan 1;251:117058. doi: 10.1016/j.carbpol.2020.117058. Epub 2020 Sep 8.
Silk fibroin (SF) is increasingly needed in tissue engineering for its superior biocompatibility. However, the practical applications of pure SF biomaterials confront bacterial infection problems. In this study, chitosan (CS) and polydopamine (PDA) were introduced into electrospun nanofibrous SF mats through layer-by-layer self-assembly (LBL) to obtain enhanced antibacterial ability and cytocompatibility. The surface morphology and composition analysis confirmed the successful deposition. After depositing 15 bilayers, the tensile modulus of the mats in wet condition increased from 2.16 MPa (pristine SF mats) to 4.89 MPa. A trend towards better hydrophilicity performance was also recorded with more bilayers coating on the mats. Besides, LBL structured mats showed improved antibacterial ability of more than 98 % against E. coli and S. aureus. In addition, advancement in biocompatibility was observed during the proliferation experiment of L929 cells. Overall, the deposition of CS and PDA may further expand the use of SF in biomedical field.
丝素蛋白（SF）具有优异的生物相容性，在组织工程中越来越受到重视。然而，纯 SF 生物材料的实际应用面临着细菌感染问题。本研究通过层层自组装（LBL）将壳聚糖（CS）和聚多巴胺（PDA）引入到静电纺丝纳米纤维 SF 基质中，以获得增强的抗菌能力和细胞相容性。表面形貌和组成分析证实了成功的沉积。沉积 15 层后，湿态下基质的拉伸模量从 2.16 MPa（原始 SF 基质）增加到 4.89 MPa。随着更多层涂层的沉积，基质的亲水性性能也呈现出更好的趋势。此外，LBL 结构的基质对大肠杆菌和金黄色葡萄球菌的抗菌能力提高了 98%以上。此外，在 L929 细胞增殖实验中观察到生物相容性的提高。总的来说，CS 和 PDA 的沉积可能会进一步扩大 SF 在生物医学领域的应用。
