Institute of Sports Biology, Shaanxi Normal University, Xi'an, China.
Shaanxi Key Laboratory of Ultrasonics, Shaanxi Normal University, Xi'an, China.
Carbohydr Polym. 2022 Jun 15;286:119278. doi: 10.1016/j.carbpol.2022.119278. Epub 2022 Feb 22.
In this research, the pharmaceutical used carboxymethyl cellulose (CMC) was successfully synthesized by ramie fiber and sodium monochloroacetate. Meanwhile, this study focused on the proliferation and differentiation of osteoblast which was stimulated by ramie based CMC. Additionally, the synergistic effects between CMC and Low-intensity pulsed ultrasound (LIPUS) for the proliferation and differentiation of osteoblast was also evaluated in this study. The experimental results demonstrated that ramie based CMC were nontoxic and cytocompatible in MC3T3-E1 cells culture; the dose of 300 μg L CMC applied in this work displayed better efficiency of promoting the osteogenic proliferation and differentiation. Meanwhile, the results were also exhibited that the combination of CMC and LIPUS (1.5 MHz, 80 mW cm) has a significant proliferation and differentiation promoting effects compared with the single factor intervention. This study provides a new pathway for bio-polymeric materials in the repair and regeneration of the bone tissues.
在这项研究中，成功地通过苎麻纤维和一氯乙酸钠合成了药用羧甲基纤维素（CMC）。同时，本研究专注于由苎麻基 CMC 刺激的成骨细胞的增殖和分化。此外，还评估了 CMC 和低强度脉冲超声（LIPUS）在促进成骨细胞增殖和分化方面的协同作用。实验结果表明，在 MC3T3-E1 细胞培养中，苎麻基 CMC 无毒且细胞相容性良好；本工作中应用的 300μg/L CMC 剂量显示出更好的促进成骨增殖和分化的效果。同时，结果还表明，CMC 和 LIPUS（1.5MHz，80mW/cm）的组合与单一因素干预相比具有显著的增殖和分化促进作用。本研究为生物聚合材料在骨组织修复和再生中的应用提供了新的途径。
