State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; International Institute of Food Innovation, Nanchang University, Nanchang 330200, China.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; International Institute of Food Innovation, Nanchang University, Nanchang 330200, China.
Food Res Int. 2022 Apr;154:111017. doi: 10.1016/j.foodres.2022.111017. Epub 2022 Feb 16.
In this study, the immunomodulation properties and the possible molecular mechanisms of mung bean skin polysaccharide (MBP) on RAW264.7 were investigated. The results showed that MBP could enhance the phagocytic activity, promote intracellular reactive oxygen species (ROS) production as well as the release of nitric oxide (NO) and cytokines in macrophages. The results of global screening of all transcripts by RNA-seq revealed 927 differentially expressed genes between the control and MBP-treated groups, including 196 up-regulated genes and 731 down-regulated genes. From GO analysis, there were 5 cellular components terms (CC), 5 biological processes terms (BP) and 10 molecular functions terms (MF). Toll-like receptor 4 (TLR4 receptor), mitogen-activated protein kinases (MAPKs) and nuclear factor-κB (NF-κB) signaling pathways were selected by KEGG analysis to elucidate the mechanism of MBP action on macrophages. Western blot analysis showed that MBP could increase the expression of TLR4, MAPKs and NF-κB signaling pathway-related proteins, which confirmed that TLR4, MAPKs and NF-κB signaling pathways were essential to MBP-mediated mocrophage activation. Moreover, the production of both NO and cytokine TNF-α was found to be significantly reduced after the addition of TLR4, MAPKs and NF-κB inhibitors. These results suggest that MBP can control the immune response of macrophages through TLR4-mediated MAPKs and NF-κB signaling pathways. This work concluded MBP could serve as a potential natural immunomodulator, providing a molecular basis for its application in functional foods.
本研究考察了绿豆皮多糖（MBP）对 RAW264.7 细胞的免疫调节作用及其可能的分子机制。结果表明，MBP 能够增强巨噬细胞的吞噬活性，促进细胞内活性氧（ROS）的产生以及一氧化氮（NO）和细胞因子的释放。通过 RNA-seq 对所有转录本进行的全局筛选显示，对照组和 MBP 处理组之间有 927 个差异表达基因，包括 196 个上调基因和 731 个下调基因。GO 分析显示，有 5 个细胞成分（CC）、5 个生物过程（BP）和 10 个分子功能（MF）术语。KEGG 分析选择 Toll 样受体 4（TLR4 受体）、丝裂原活化蛋白激酶（MAPKs）和核因子-κB（NF-κB）信号通路来阐明 MBP 对巨噬细胞作用的机制。Western blot 分析表明，MBP 能够增加 TLR4、MAPKs 和 NF-κB 信号通路相关蛋白的表达，这证实了 TLR4、MAPKs 和 NF-κB 信号通路是 MBP 介导的巨噬细胞激活所必需的。此外，添加 TLR4、MAPKs 和 NF-κB 抑制剂后，NO 和细胞因子 TNF-α 的产生均明显减少。这些结果表明，MBP 可以通过 TLR4 介导的 MAPKs 和 NF-κB 信号通路来控制巨噬细胞的免疫反应。本研究表明 MBP 可以作为一种潜在的天然免疫调节剂，为其在功能性食品中的应用提供了分子基础。
