Cullmann W, Dick W
Immun Infekt. 1986 Sep;14(5):188-90.
A simple enzymatic assay for simultaneous determinations of amino-/ureidopenicillins and clavulanic acid concentrations in various body fluids is described; no pretreatment of the samples is required. The assay is based on the competitive inhibition of the chromogenic compound Padac by clavulanic acid in the presence of Proteus vulgaris beta-lactamase. Detection limit for clavulanic acid amounts 0.02 mg/l. Interferences with other antibiotics such as aminoglycosides, macrolides, quinolones etc. can be ruled out.
本文描述了一种简单的酶法，用于同时测定各种体液中氨基/脲基青霉素和克拉维酸的浓度；无需对样品进行预处理。该测定基于在普通变形杆菌β-内酰胺酶存在下，克拉维酸对显色化合物Padac的竞争性抑制作用。克拉维酸的检测限为0.02mg/l。可以排除与其他抗生素如氨基糖苷类、大环内酯类、喹诺酮类等的干扰。
