Grupo de Investigación en Reumatología (GIR), Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, Universidade da Coruña (UDC), C/ As Xubias de Arriba 84, 15006, A Coruña, Spain.
Facultad de Medicina, Universidad Autónoma de Coahuila, Torreon Coahuila, Mexico.
Sci Rep. 2022 Mar 25;12(1):5173. doi: 10.1038/s41598-022-09265-y.
To evaluate the influence of mitochondrial DNA haplogroups on the risk of knee OA in terms of their interaction with obesity, in a population from Mexico. Samples were obtained from (n = 353) knee OA patients (KL grade ≥ I) and (n = 364) healthy controls (KL grade = 0) from Mexico city and Torreon (Mexico). Both Caucasian and Amerindian mtDNA haplogroups were assigned by single base extension assay. A set of clinical and demographic variables, including obesity status, were considered to perform appropriate statistical approaches, including chi-square contingency tables, regression models and interaction analyses. To ensure the robustness of the predictive model, a statistical cross-validation strategy of B = 1000 iterations was used. All the analyses were performed using boot, GmAMisc and epiR package from R software v4.0.2 and SPSS software v24. The frequency distribution of the mtDNA haplogroups between OA patients and healthy controls for obese and non-obese groups showed the haplogroup A as significantly over-represented in knee OA patients within the obese group (OR 2.23; 95% CI 1.22-4.05; p-value = 0.008). The subsequent logistic regression analysis, including as covariate the interaction between obesity and mtDNA haplogroup A, supported the significant association of this interaction (OR 2.57; 95% CI 1.24-5.32; p-value = 0.011). The statistical cross-validation strategy confirmed the robustness of the regression model. The data presented here indicate a link between obesity in knee OA patients and mtDNA haplogroup A.
为了评估线粒体 DNA 单倍群在肥胖影响膝关节骨关节炎风险方面的作用，在墨西哥人群中进行了研究。样本来自墨西哥城和托雷翁的 353 名膝关节骨关节炎患者（KL 分级≥I）和 364 名健康对照者（KL 分级=0）。通过单碱基延伸法检测高加索人和美洲印第安人 mtDNA 单倍群。考虑了一系列临床和人口统计学变量，包括肥胖状况，以进行适当的统计方法，包括卡方检验、回归模型和交互分析。为了确保预测模型的稳健性，使用了 B=1000 次迭代的统计交叉验证策略。所有分析均使用 R 软件 v4.0.2 中的 boot、GmAMisc 和 epiR 包和 SPSS 软件 v24 进行。在肥胖和非肥胖组中，OA 患者和健康对照组之间 mtDNA 单倍群的频率分布显示，肥胖组中 haplogroup A 在膝关节骨关节炎患者中明显过度表达（OR 2.23；95%CI 1.22-4.05；p 值=0.008）。随后的包括肥胖和 mtDNA haplogroup A 之间交互作用的协方差的逻辑回归分析支持了这种交互作用的显著相关性（OR 2.57；95%CI 1.24-5.32；p 值=0.011）。统计交叉验证策略证实了回归模型的稳健性。这里呈现的数据表明，膝关节骨关节炎患者的肥胖与 mtDNA haplogroup A 之间存在联系。
