ICAR-National Institute of High Security Animal Diseases, Bhopal, India.
North Eastern Regional Disease Diagnostic Laboratory, Guwahati, India.
Transbound Emerg Dis. 2022 Sep;69(5):e2020-e2027. doi: 10.1111/tbed.14536. Epub 2022 Apr 9.
African swine fever (ASF), considered as the most dreadful swine disease due to its very high mortality, emerged in India in 2020. The complete genome analysis of ASF viruses isolated during the first outbreaks in India showed a few unique non-synonymous mutations in MGF 369-11L, MGF 505-4R, K205R and B263R genes. Frame shifts in the protein coding sequences were observed in DP60R, ASFV-G_ACD 00190, MGF 110-10-L-MGF110-14L fusion, MGF 360-14L and I267L genes of Indian ASF viruses as compared to ASFV/Georgia/2007. Complete genome based phylogenetic analysis of p72-genotype-II viruses showed the clustering of Indian isolates with ASFV/Wuhan/2019 in a separate clade. Phylogenetic analysis of concatenated sequences of 14 open reading frames (ORF) having single nucleotide polymorphisms (SNP) showed distinct grouping of Indian ASFVs with other Asian ASFVs. This is the first complete genome characterization of ASF viruses isolated from domestic pigs in India. The results indicate that number of Tandem Repeat Sequence (TRS) in the intergenic region between I73R and I329L genes, and the 14 ORFs with SNP reported in this study could be the genetic determinants to differentiate the closely related p72-genotype II viruses circulating in Asia.
非洲猪瘟（ASF），因其极高的死亡率而被认为是最可怕的猪病，于 2020 年在印度出现。对印度首次暴发期间分离的 ASF 病毒的全基因组分析显示，MGF 369-11L、MGF 505-4R、K205R 和 B263R 基因中存在一些独特的非同义突变。与 ASFV/Georgia/2007 相比，印度 ASF 病毒的 DP60R、ASFV-G_ACD 00190、MGF 110-10-L-MGF110-14L 融合、MGF 360-14L 和 I267L 基因中观察到蛋白质编码序列的移码。基于 p72-基因型 II 病毒全基因组的系统进化分析表明，印度分离株与 ASFV/Wuhan/2019 聚在一个单独的分支中。14 个开放阅读框（ORF）的串联序列的系统进化分析显示，印度 ASFVs 与其他亚洲 ASFVs 明显分组。这是首次对印度从家猪中分离的 ASF 病毒进行全基因组特征描述。结果表明，I73R 和 I329L 基因之间的基因间区中的串联重复序列（TRS）数量，以及本研究报道的 14 个具有 SNP 的 ORF 可能是区分在亚洲循环的密切相关的 p72-基因型 II 病毒的遗传决定因素。
