从液态粪便中分离出的与溶组织内阿米巴相关的迪斯帕内阿米巴感染患者的微小RNA表达

MicroRNAs expression of patients infected with Entamoeba histolytica in association with Entamoeba dispar isolated from liquid stool.

作者信息

Ahmad Neama Ali

机构信息

Department of Biology, College of Science, University of Kirkuk, Iraq.

出版信息

Ann Parasitol. 2021;67(4):805-812. doi: 10.17420/ap6704.399.

DOI:10.17420/ap6704.399

PMID:35339120

Abstract

Amoebic dysentery is a parasitic intestinal infection that causes diarrhea containing blood or mucus. A total of 100 amoebic stool samples were collected from patients attending Azadi Hospital in Kirkuk City, Iraq during the period from 1st December 2020 to 15th June 2021. The results showed that out of 100 samples of amoebic infected patients, 88 (88%) were infected with E. histolytica, while 12 (12%) were infected with E. dispar. There was a nonsignificant difference between males 46 (46.0%) and females 42 (42%) among E. histolytica infected patients, and between males 7 (7%) and females 5 (5%) among E. dispar infected patients. According to age groups, the majority of the samples 39 (39%) were reported at the age group 15-44 years, while the lower number 14 (14%) was recorded in the age group 1-4 years. The results of distribution of Entamoeba stages showed that the distribution rate of E. histolytica trophozoite stage was 25 (92.6%), E. histolytica cyst stage was 49 (86%) and both of trophozoite + cyst stages was 14 (87.5%), while the distribution rate of E. dispar trophozoite stage was 2 (7.4%), cyst 8 (14%) and the trophozoite + cyst stages was 2 (7.4%), with a highly significant difference (P=0.002). The count of RBCs per field for each Entamoeba cell showed that the RBCs were found only in E. histolytica infection, while they were not found in E. dispar infection with a highly significant difference (P<0.01). Results of observing bacterial activity under microscope showed that there was no relationship between Entamoeba infections and bacterial infections (P>0.05). Out of 60 stool samples from amoebic patients, 17 (28.3%) were positive for microRNA-21 of E. histolytica with a mean of (8.30±13.34), while 0 (0.00%) of E. dispar showed a mean of (1.51±1.91), (P=0.005). Out of 60 stool sample from amoebic patients, 27 (45.0%) were positive for microRNA-22 of E. histolytica with a mean of (44.31±61.43), while 0 (0.00%) of E. dispar showed the mean (1.43±3.33), (P=0.005).

摘要

阿米巴痢疾是一种寄生虫性肠道感染，会导致腹泻，粪便中含有血液或黏液。在2020年12月1日至2021年6月15日期间，从伊拉克基尔库克市阿扎迪医院就诊的患者中总共采集了100份阿米巴粪便样本。结果显示，在100份阿米巴感染患者样本中，88份（88%）感染了溶组织内阿米巴，而12份（12%）感染了迪斯帕内阿米巴。在溶组织内阿米巴感染患者中，男性46例（46.0%）和女性42例（42%）之间无显著差异；在迪斯帕内阿米巴感染患者中，男性7例（7%）和女性5例（5%）之间也无显著差异。按年龄组划分，大多数样本39份（39%）来自15 - 44岁年龄组，而数量较少的14份（14%）来自1 - 4岁年龄组。溶组织内阿米巴各阶段分布结果显示，溶组织内阿米巴滋养体阶段分布率为25份（92.6%），包囊阶段为49份（86%），滋养体 + 包囊阶段为14份（87.5%）；而迪斯帕内阿米巴滋养体阶段分布率为2份（7.4%），包囊8份（14%），滋养体 + 包囊阶段为2份（7.4%），差异具有高度显著性（P = 0.002）。每个溶组织内阿米巴细胞视野中的红细胞计数显示，红细胞仅在溶组织内阿米巴感染中发现，而在迪斯帕内阿米巴感染中未发现，差异具有高度显著性（P < 0.01）。显微镜下观察细菌活性的结果显示，溶组织内阿米巴感染与细菌感染之间无关联（P > 0.05）。在60份阿米巴患者粪便样本中，17份（28.3%）溶组织内阿米巴的微小RNA - 21呈阳性，平均值为（8.30±13.34），而迪斯帕内阿米巴为0份（0.00%），平均值为（1.51±1.91），（P = 0.005）。在60份阿米巴患者粪便样本中，27份（45.0%）溶组织内阿米巴的微小RNA - 22呈阳性，平均值为（44.31±61.43），而迪斯帕内阿米巴为0份（0.00%），平均值为（1.43±3.33），（P = 0.005）。