Departments of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA,
Departments of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA.
J Zoo Wildl Med. 2022 Mar;53(1):100-107. doi: 10.1638/2021-0028.
The moon jellyfish () is a scyphozoan frequently maintained in public and private aquaria. Little research has been conducted to investigate the effects of various drugs, such as anesthetics, in this species. Tricaine methanesulfonate (MS-222), a common immersion anesthetic for fish and amphibians, was evaluated in a managed population of moon jellyfish. Twenty-four clinically healthy jellyfish were assigned into three groups of eight for trials of 0.3 g/L MS-222 (low concentration [LC]), 0.6 g/L MS-222 (high concentration [HC]), and a saltwater control. The goal was to evaluate the effects of MS-222 administration on moon jellyfish movement and response to stimuli. Movement and response to stimuli were measured via rocking and probe stimulus tests and observations of bell contraction quality and body tone. These tests were performed at baseline and throughout both drug exposure and recovery periods. A threshold drug effect was defined based on systematic scoring criteria. Additionally, elastomer tags were administered to four of eight animals in each MS-222 group to evaluate response to tag placement after drug exposure. Threshold drug effect was achieved in six of eight individuals in the LC group and eight of eight individuals in the HC group. The LC group had median threshold and recovery times of 12.2 and 10.1 min, respectively, while the HC group had median threshold and recovery times of 4.0 and 19.9 min, respectively. The HC group had significantly faster time to threshold drug effect ( < 0.001) and longer recovery times (= 0.005) than the LC group. In both the LC and HC tagged group, three of four jellyfish had no reaction to tag placement. All animals recovered uneventfully, and there were no mortalities. MS-222 at 0.3 and 0.6 g/L decreased movement and response to stimuli in moon jellyfish.
海月水母（）是一种经常在公共和私人水族馆中饲养的钵水母纲动物。目前，针对这种物种，人们对各种药物（如麻醉剂）的影响研究甚少。甲磺酸间甲苯胺（MS-222）是一种常用于鱼类和两栖类动物的浸浴麻醉剂，本研究评估了其在管理种群的海月水母中的效果。将 24 只临床健康的水母分为三组，每组 8 只，分别接受 0.3 g/L MS-222（低浓度[LC]）、0.6 g/L MS-222（高浓度[HC]）和盐水对照处理。目的是评估 MS-222 给药对海月水母运动和对刺激的反应的影响。通过晃动和探针刺激测试以及观察钟形收缩质量和身体色调来测量运动和对刺激的反应。这些测试在基线以及药物暴露和恢复期间进行。根据系统评分标准定义了药物作用的阈值。此外，在每组 MS-222 中的 4 只动物上给予弹性体标签，以评估药物暴露后对标签放置的反应。LC 组中 6 只个体和 HC 组中 8 只个体达到了阈值药物效应。LC 组的中位阈值和恢复时间分别为 12.2 分钟和 10.1 分钟，而 HC 组的中位阈值和恢复时间分别为 4.0 分钟和 19.9 分钟。HC 组达到阈值药物效应的时间明显快于 LC 组（<0.001），恢复时间也更长（=0.005）。在 LC 和 HC 标记组中，有 3 只水母对标签放置没有反应。所有动物均恢复良好，无死亡。0.3 和 0.6 g/L 的 MS-222 降低了海月水母的运动和对刺激的反应。
