Centre for Crop Disease Management, Curtin University, Bentley, WA 6102, Australia; and Commonwealth Scientific and Industrial Research Organization, Agriculture and Food, Floreat, WA 6913, Australia.
School of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide, Urrbrae, SA 5064, Australia.
Funct Plant Biol. 2022 Jun;49(7):634-646. doi: 10.1071/FP21216.
Sclerotinia stem rot (SSR), caused by Sclerotinia sclerotiorum , is one of the most economically devastating diseases in chickpea (Cicer arietinum L.). No complete resistance is available in chickpea to this disease, and the inheritance of partial resistance is not understood. Two hundred F7 recombinant inbred lines (RILs) derived from a cross between a partially resistant variety PBA HatTrick, and a highly susceptible variety Kyabra were characterised for their responses to SSR inoculation. Quantitative trait locus (QTL) analysis was conducted for the area under the disease progress curve (AUDPC) after RIL infection with S. sclerotiorum . Four QTLs on chromosomes, Ca4 (qSSR4-1, qSSR4-2), Ca6 (qSSR6-1) and Ca7 (qSSR7-1), individually accounted for between 4.2 and 15.8% of the total estimated phenotypic variation for the response to SSR inoculation. Candidate genes located in these QTL regions are predicted to be involved in a wide range of processes, including phenylpropanoid biosynthesis, plant-pathogen interaction, and plant hormone signal transduction. This is the first study investigating the inheritance of resistance to S. sclerotiorum in chickpea. Markers associated with the identified QTLs could be employed for marker-assisted selection in chickpea breeding.
茎基溃疡病（SSR）是由核盘菌引起的，是鹰嘴豆（Cicer arietinum L.）上最具经济破坏性的疾病之一。鹰嘴豆对这种疾病没有完全抗性，部分抗性的遗传也不清楚。从部分抗性品种 PBA HatTrick 和高度敏感品种 Kyabra 杂交的 200 个 F7 重组自交系（RIL）对 SSR 接种的反应进行了特征描述。对 RIL 感染核盘菌后的病害进展曲线（AUDPC）进行了数量性状位点（QTL）分析。在 Ca4（qSSR4-1、qSSR4-2）、Ca6（qSSR6-1）和 Ca7（qSSR7-1）染色体上的 4 个 QTL 分别解释了 SSR 接种反应的总表型变异的 4.2%至 15.8%。这些 QTL 区域中的候选基因被预测参与了广泛的过程，包括苯丙烷生物合成、植物-病原体相互作用和植物激素信号转导。这是首次研究鹰嘴豆对核盘菌抗性的遗传。与鉴定的 QTL 相关的标记可用于鹰嘴豆育种中的标记辅助选择。
